Internalization and Colocalization of a Polyclonal Antibody Against Porphyromonas gingivalis FimA type I in Infected Cells.

Objective:  The aim of this work was to investigate the effect of a rabbit polyclonal antibody specific to FimA type I (FimI) protein internalized into infected cells.

Materials And Methods:  Rabbits were immunized with FimI protein and the serum was collected for immunoglobulin (Ig) purification. For visualization of the antibody inside the cells, it was labeled with Cy3 dye.

Morus alba Stem Extract Suppresses Matrix Metalloproteinases (MMP)-1, MMP-9, and Tissue Inhibitors of Metalloproteinase (TIMP)-1 Expression via Inhibition of IκBα Degradation Induced by Porphyromonas gingivalis LPS Signal in THP-1 Cells.

Objectives: The aim of this study is to evaluate the inhibitory effects of stem extract (MSE) on the expression of matrix metalloproteinases (MMP)-1, MMP-9, and tissue inhibitors of metalloproteinase (TIMP)-1 in lipopolysaccharide (LPS)-activated-acute monocytic leukemia cell line (THP-1).

Materials And Methods: THP-1 cells were treated with noncytotoxic concentrations of MSE combined with 1 µg/mL of LPS. The mRNA levels of MMP-1, MMP-9, and TIMP-1 were evaluated via quantitative real-time polymerase chain reaction.

Antibacterial and Anti-inflammatory Potential of Stem Extract.

Background: Periodontitis, a chronic inflammatory disease, is the leading cause of tooth loss in adults. Evidence for the anti inflammatory activity of Stem Extract (MSE) in periodontal disease is limited.

Objective: The study aimed to investigate the inhibitory effect of MSE on the growth of periodontopathic bacteria and expression of interleukin (IL)-6 and IL-8 in Lipopolysaccharide (LPS)-stimulated human Periodontal Ligament (hPDL) fibroblasts.

Apoptotic effect of alpha-mangostin on head and neck squamous carcinoma cells.

Objective: The purposes of this study were to measure the cytotoxic effect of alpha-mangostin on head and neck squamous cell carcinoma (HNSCC) cell lines, to evaluate the apoptotic aspect of dead cells, and to identify the molecular mechanism involved in apoptosis.

Methods: The human HNSCC cell lines HN-22, HN-30 and HN-31 were treated with alpha-mangostin. The apoptotic effects of alpha-mangostin on HNSCC cells were determined by observation the morphological changes of cells, immunofluorescence for single-stranded DNA (ssDNA), and DNA fragmentation analysis.

Antifungal activity of alpha-mangostin against Candida albicans.

This study was conducted to examine the activity of alpha-mangostin against Candida albicans, the most important microorganism implicated in oral candidiasis. Its activity was compared to Clotrimazole and Nystatin. Results showed that alpha-mangostin was effective against C.