It is imperative that promising bacterial cellulose-producing bacteria mainly belongs to genera Acetobacter (acid-producing bacteria). In order to screen cellulose-producing Acetobacter, the isolated cultures from vinegar/rotten fruits were inoculated in Hestrin-Schramm (HS) medium containing ethanol and CaCO. After the desired incubation, the positive cultures form a zone, which is observed around the bacterial growth, resulted from the solubilization of CaCO by acetic acid produced from the oxidation of ethanol during fermentation.
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