The clinical application of affected-embryo-based SNP haplotype analysis for patients with de novo pathogenic mutations in PGT-M cycles.

Purpose: In preimplantation genetic testing for monogenic/single gene disorders (PGT-M) cycles, direct detection of the pathogenic mutation combined with indirect haplotype analysis are recommended to achieve accurate diagnosis. However, it poses a challenge to conduct haplotype analysis for patients carried de novo pathogenetic mutations or without no identified haplotype in families. Herein, the strategy of affected-embryo-based haplotype analysis was implemented in clinical practice to provide a convenient, economical and effective way for such patients.

Effect of fibroblasts small- conductance Ca -activated potassium channel subtype 2 (SK2) on myocardial fibrosis in pressure overload mouse.

Studies have shown that Small conductance Ca2 + -activated K+ (SK) channel are expressed in fibroblasts. We aimed to determine the expression of SK2 channels in cardiac fibroblasts during myocardial hypertrophy and investigate its relationship with fibrotic remodeling. Myocardial hypertrophy and fibrotic remodeling induced by transverse aortic constriction (TAC) were assessed by echocardiography, Masson's trichrome staining and Western blot.

Evidence of Spin Density Waves in La_{3}Ni_{2}O_{7-δ}.

The recently discovered superconductivity with critical temperature T_{c} up to 80 K in the double-layer Nickelate La_{3}Ni_{2}O_{7-δ} under pressure has drawn great attention. Here, we report the positive muon spin relaxation (μ^{+}SR) study of polycrystalline La_{3}Ni_{2}O_{6.92} under ambient pressure.

Application of quantitative pharmacology analysis to support early clinical development of oncology drugs: dose selection.

The selection of appropriate starting dose and suitable method to predict an efficacious dose for novel oncology drug in the early clinical development stage poses significant challenges. The traditional methods of using body surface area transformation from toxicology studies to predict the first-in human (FIH) starting dose, or simply selecting the maximum tolerated dose (MTD) or maximum administered dose (MAD) as efficacious dose or recommended phase 2 dose (RP2D), are usually inadequate and risky for novel oncology drugs.Due to the regulatory efforts aimed at improving dose optimisation in oncology drug development, clinical dose selection is now shifting away from these traditional methods towards a comprehensive benefit/risk assessment-based approach.

Effect of anesthesia mode during endovascular treatment on neurological functional outcomes in patients with acute posterior circulation stroke.

Objectives: To compare the effect of anesthesia mode on the neurological functional outcomes in patients undergoing endovascular treatment for acute posterior circulation ischemic stroke.

Methods: Clinical data of 656 patients undergoing intravascular therapy for acute posterior circulation ischemic stroke registered in online Acute Stroke Patients for Stroke Management Quality Evaluation Database from January 2017 to December 2022 were retrospectively analyzed. The data included 163 cases with conscious sedation and 493 cases with general anesthesia during the procedure.

Dual-Confinement Effect of Nanocages@Nanotubes Suppresses Polysulfide Shuttle Effect for High-Performance Lithium-Sulfur Batteries.

The shuttle effect of lithium polysulfides (LiPSs) severely hinders the development and commercialization of lithium-sulfur batteries, and the design of high-conductive carbon fiber-host material has become a key solution to suppress the shuttle effect. In this work, a unique Co/CoN-carbon nanocages@TiO-carbon nanotubes structure (NC@TiO-CNTs) is constructed using an electrospinning and nitriding process. Lithium-sulfur batteries using NC@TiO-CNTs as cathode host materials exhibit high sulfur utilization (1527 mAh g at 0.

MGA-seq: robust identification of extrachromosomal DNA and genetic variants using multiple genetic abnormality sequencing.

Genomic abnormalities are strongly associated with cancer and infertility. In this study, we develop a simple and efficient method - multiple genetic abnormality sequencing (MGA-Seq) - to simultaneously detect structural variation, copy number variation, single-nucleotide polymorphism, homogeneously staining regions, and extrachromosomal DNA (ecDNA) from a single tube. MGA-Seq directly sequences proximity-ligated genomic fragments, yielding a dataset with concurrent genome three-dimensional and whole-genome sequencing information, enabling approximate localization of genomic structural variations and facilitating breakpoint identification.

Influence of titanate photocatalyst in biohydrogen yield via photo fermentation from corn stover.

The effects of three common titanate photocatalysts (TPC) on the photo fermentation biohydrogen production (PFHP) from corn stover were studied in this paper. Compared with CaTiO and BaTiO, the experimental group with the addition of MgTiO showed stronger potential for PFHP, the maximum hydrogen yield of 344 mL (68.8 mL/g TS) was obtained at 3 g/L MgTiO, increased by 48.

The effect of the day 3 embryo cell number on the neonatal outcomes of day 5 single blastocyst transfer in frozen embryo transfer cycles.

Objective: To evaluate the influence of the day 3 embryo cell number on the neonatal outcomes of day 5 single blastocyst transfer in frozen embryo transfer (FET) cycles.

Methods: This retrospective study analysed a total of 2315 delivery cycles of day 5 single blastocyst transfer in FET cycles, including 489, 761 and 1103 live-born infants segregated according to a day 3 embryo cell number of <8, 8 and >8 cells, respectively. The neonatal outcomes of the three groups were compared.

Estimating the potential of biohydrogen production and carbon neutralization contribution from crop straw.

In order to achieve the carbon neutrality goal set by Chinese government, the potential contribution of hydrogen production from crop residues by microbial fermentation technology and Greenhouse gas (GHGs) reduction have been studied. Firstly, the annual yield of crop straw was estimated according to crop yield and grass grain ratio, and then the grey model GM (1, 1) was applied to predict the crop residues resources available for hydrogen production in various provinces in China in 2021. The results showed that the maximum resource of straw being available for hydrogen production is about 4.

The influence of day 3 embryo cell number on the clinical pregnancy and live birth rates of day 5 single blastocyst transfer from frozen embryo transfer cycles.

Background: To evaluate the influence of day 3 embryo cell number on the clinical pregnancy and live birth rates of day 5 single blastocyst transfer in frozen embryo transfer (FET) cycles.

Methods: Our retrospective study included 3761 day 5 single blastocyst FET cycles between January 2015 and December 2019. These FET cycles were divided into three groups according to the day 3 embryo cell number: 939 cycles in the < 8-cell group, 1224 cycles in the 8-cell group and 1598 cycles in the > 8-cell group.

Elevated serum progesterone levels on the hCG trigger day have a negative impact on the live birth rate in the first fresh IVF-ET cycle.

To investigate whether there is a relationship between elevated serum progesterone (PROG) on the hCG trigger day and the live birth rate (LBR) in IVF/ICSI cycles, the retrospective analysis was carried out from the patients undergoing the first ART cycles throughout 2016. The PROG levels were measured on the hCG trigger day. The LBR, clinical pregnancy rate (CPR), implantation rate (IR) and other parameter rate values were compared among the three different PROG elevations.

Circular RNA circFoxo3 Promotes Granulosa Cell Apoptosis Under Oxidative Stress Through Regulation of FOXO3 Protein.

Oxidative stress leads to ovarian functional decline by inducing granulosa cell (GC) apoptosis. Circular RNA circFoxo3 acts as a critical factor in regulating cell cycle and apoptosis, and cellular senescence in tumor cells. However, function of circFoxo3 is little understood in oxidative stress-induced injury of follicular GCs.

Influence Factors and Predictive Models for the Outcome of Patients with Ischemic Stroke after Intravenous Thrombolysis: A Multicenter Retrospective Cohort Study.

Objective: Intravenous thrombolysis (IVT) is currently the main effective treatment for patients with ischemic stroke. This study aimed to analyze the factors affecting the early neurological recovery and prognosis of thrombolytic therapy after surgery and to construct predictive models.

Materials And Methods: A total of 849 patients with ischemic stroke who received IVT treatment at six centers from June 2017 to March 2021 were included.

Primary stent implantation for bilateral spontaneous cervical ICA dissections with hypoperfusion after 72 h from onset: a case report.

Background: Spontaneous cervical internal carotid artery dissection (cICAD) is a common cause of stroke in young adults. Endovascular therapy is an indispensable treatment for cICAD in some cases, but it faces great challenges.

Case Presentation: A bilateral spontaneous cICADs with hypoperfusion-related AIS after 72 h from the onset was presented herein.

Non-invasive embryo selection strategy for clinical IVF to avoid wastage of potentially competent embryos.

Research Question: Can a non-invasive embryo transfer strategy provide a reference for embryo selection to be established?

Design: Chromosome sequencing of 345 paired blastocyst culture medium and whole blastocyst samples was carried out and a non-invasive embryo grading system was developed based on the random forest machine learning algorithm to predict blastocyst ploidy. The system was validated in 266 patients, and a blinded prospective observational study was conducted to investigate clinical outcomes between machine learning-guided and traditional non-invasive preimplantation genetic testing for aneuploidy (niPGT-A) analyses. Embryos were graded as A, B or C according to their euploidy probability levels predicted by non-invasive chromosomal screening (NICS).

Enhancement effect of defoamer additives on photo-fermentation biohydrogen production process.

Foaming is a key issue should be solved in the process of photo-fermentation biohydrogen production (PFHP), since it has negative influence on the hydrogen yield potential, especially when taken straw as substrate. Appropriate foam control measures must be considered for industrialization. Hence, in this work, foam height and biohydrogen yield were selected as index, the effect of defoamer addition on PFHP was investigated.

Blastocyst development rate influences singleton gestational age of similarly graded blastocysts after vitrified-warmed single embryo transfer cycles.

Research Question: Does the development rate of blastocysts influence neonatal outcomes after blastocyst transfer cycles when the morphological score of the transferred blastocysts is similar?

Design: A retrospective study involving singleton live births born to 1280 women undergoing single frozen blastocyst transfers (FBTs) between January 2016 and December 2018 at a tertiary care centre. Patients were grouped into day-5 or day-6 groups depending on the development rate of blastocysts. These were further grouped into four groups based on the blastocyst inner cell mass and trophectoderm scoring: excellent (AA); good (AB or BA); average (AC, CA or BB); and poor (BC or CB).

Chitinase Chi 2 Positively Regulates Cucumber Resistance against f. sp. cucumerinum.

Cucumber ( L.) is an important vegetable crop worldwide, and wilt (), caused by f. sp.

Enhancing photo-fermentative biohydrogen production using different zinc salt additives.

The kinetic properties of the hydrogen yield of photosynthetic bacteria were investigated using Han-Levenspiel and modified Gompertz models to determine the effects of different zinc salts on the growth and hydrogen production of the photosynthetic bacterium HAU-M1. Inorganic zinc salts (zinc standard solution and zinc sulfate) inhibited bacterial growth by 1-4-fold higher than organic zinc salts (zinc lactate and zinc gluconate). Among these four zinc salts, 5 mg/L zinc lactate displayed the weakest inhibition performance.

Enhancing photo-fermentation biohydrogen production from corn stalk by iron ion.

This study aimed to investigate the enhancement of iron ion on growth, metabolic pathway, and biohydrogen production performance of biohydrogen producing bacteria HAU-M1. Different concentrations of Fe and Fe were respectively added into fermentation broth of photo-fermentation biohydrogen production (PFHP) from corn stalk. Regular sampling test was used to measure the characteristics of fermentation broth and gas, metabolic pathway, energy conversion efficiency, and kinetic of PFHP.

Identification of Susceptibility Genes for in Cucumber via Comparative Proteomic Analysis.

wilt () in cucumber ( L.), caused by f. sp.

Identification of miRNA-Target Gene Pairs Responsive to Wilt of Cucumber via an Integrated Analysis of miRNA and Transcriptome Profiles.

wilt () of cucumber ( L.) caused by f. sp.