Ultrasensitive Electrochemical Biosensors Based on Allosteric Transcription Factors (aTFs) for Pb Detection.

Exposure to Pb in the environment, especially in water, poses a significant threat to human health and urgently necessitates the development of highly sensitive Pb detection methods. In this study, we have integrated the high sensitivity of electrochemical techniques with allosteric transcription factors (aTFs) to develop an innovative electrochemical biosensing platform. This biosensors leverage the specific binding and dissociation of DNA to the aTFs (PbrR) on electrode surfaces to detect Pb.