First Report of Causing Pythium Damping-off and Root Rot on Pepper in Guizhou, China.

Pepper ( L.) is a popular vegetable and condiment consumed around the world. In the Guizhou Province of China, peppers are the most commonly grown crop on 300,000 planted hectares.

A simple graphene oxide-based DNA purification strategy for plant pathogen detection.

Background: The on-site molecular detection of plant pathogens is particularly important for the development of sustainable agriculture. Extracting DNA from plant tissues, microbes or coexisting environments is complex, labor-intensive and time-consuming. To facilitate this process, we propose a DNA purification strategy based on graphene oxide (GO).

Host Range and Loop-Mediated Isothermal Amplification Detection of from Guizhou, China.

, especially , causes devastating root rot, blight, and other diseases in various species of cash crops. To investigate the distribution and host range of in Guizhou, a suitable habitat for this pathogen, we collected 156 root-diseased samples, isolated the pathogens, and found that is widespread and has eleven host plants, including four novel hosts. Furthermore, to effectively identify , we developed a simple and dependable method based on loop-mediated isothermal amplification (LAMP), which used a primer set designed from the internal transcribed spacer sequences with high specificity and sensitivity of 1 pg/μL.