Publications by authors named "Ninghua Liu"

Although the technologies for auricular reconstruction in microtia have improved, issues such as low hairlines or excessive hair growth can still pose aesthetic problems for the reconstructed ear. Laser depilation has been reported as a solution for hair problems. However, few studies have discussed the appropriate region for hair removal.

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The deep marine sediments represent a major repository of organic matter whilst hosting a great number of uncultivated microbes. Microbial metabolism plays a key role in the recycling of organic matter in the deep marine sediments. D-amino acids (DAAs) and DAA-containing muropeptides, an important group of organic matter in the deep marine sediments, are primarily derived from bacterial peptidoglycan decomposition.

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Objectives: This study aims to evaluate the use of 755-nm picosecond alexandrite laser with a focus lens array to treat facial pores.

Methods: Laser treatment was performed on 129 patients between January 2021 and October 2022. VISIA imaging system was used for photographic assessments, the total average number and pore index was calculated, the physicians' assessment score and patient satisfaction score were collected, and the incidence of disadvantage effects was also documented.

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Marine bacteria play important roles in the degradation and cycling of algal polysaccharides. However, the dynamics of epiphytic bacterial communities and their roles in algal polysaccharide degradation during kelp decay are still unclear. Here, we performed metagenomic analyses to investigate the identities and predicted metabolic abilities of epiphytic bacterial communities during the early and late decay stages of the kelp .

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Article Synopsis
  • Marine bacteria, particularly those from the SAR92 clade, can break down dimethylsulfoniopropionate (DMSP), impacting the global sulfur cycle and climate.
  • The research identified two main pathways for DMSP degradation, involving DMSP lyase (DddD) and DMSP demethylase (DmdA), which produce gases like dimethylsulfide and methanethiol.
  • The findings highlight the widespread presence of SAR92 bacteria in oceans and their significance as DMSP degraders and climate-active gas sources, enhancing our understanding of oligotrophic bacteria's roles in marine environments.
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Nitrogen (N) is an essential element for microbial growth and metabolism. The growth and reproduction of microorganisms in more than 75% of areas of the ocean are limited by N. Prochlorococcus is numerically the most abundant photosynthetic organism on the planet.

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A Gram-stain-negative, aerobic, flagellated, and long rod-shaped bacterium, designated strain SM1973, was isolated from an intertidal sediment sample collected from the coast of Qingdao, PR China. Strain SM1973 grew at 15-37 °C and with 0-5.5 % NaCl.

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Hearing improvement is another basic requirement for microtia patients in addition to aesthetic needs. This quantitative framework fabrication method can reduce the learning curve, obtain satisfactory aesthetic results with few complications, and reserve a certain space for future canalplasty. Laryngoscope, 134:148-153, 2024.

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Article Synopsis
  • Braun's lipoprotein (Lpp) is crucial for maintaining the stability of the cell envelope by linking the outer membrane to the peptidoglycan layer.
  • Researchers used atomic force microscopy to observe Lpp directly on peptidoglycan sacculi, finding it evenly spread across the surface but absent at the division site during cell division.
  • The study provides a method for better understanding the distribution of Lpp and other proteins bound to the peptidoglycan layer through direct imaging techniques.
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Diaminopimelic acid (DAP) is a unique component of the cell wall of Gram-negative bacteria. It is also an important component of organic matter and is widely utilized by microbes in the world's oceans. However, neither DAP concentrations nor marine DAP-utilizing microbes have been investigated.

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Background And Aims: The effect of ginsenoside Rb1 on D-galactosamine (D-GalN)/lipopolysaccharide (LPS)-induced acute liver injury (ALI) is unknown. The aim of this study was to evaluate the effect of ginsenoside Rb1 on ALI and its underlying mechanisms.

Methods: Mice were pretreated with ginsenoside Rb1 by intraperitoneal injection for 3 days before D-GalN/LPS treatment, to induce ALI.

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Methods: Wound-healing assay and Transwell assay were utilized to evaluate the effect of ginsenoside Rb1 on the migration of BMSCs. RT-PCR and Western blotting were performed to evaluate the expression of stromal-derived factor 1 (SDF-1), C-X-C chemokine receptor type 4 (CXCR4), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (PKB; AKT).

Results: Ginsenoside Rb1 significantly enhanced the migration of BMSCs through the activation of SDF-1, CXCR4, p-PI3K/PI3K, and p-Akt/Akt relative expression.

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Article Synopsis
  • The Mariana Trench, the deepest part of the ocean, has extreme conditions like high pressure, cold temperatures, and no light, which makes it challenging for life.
  • Researchers studied the complete genome of Marinomonas profundi M1K-6, a microorganism found in the trench, revealing its adaptations for surviving these harsh conditions, including genes for cold tolerance and ion transport.
  • The findings enhance our understanding of how Marinomonas spp. thrive in deep-sea environments that are low in oxygen and nutrients, highlighting their unique survival strategies.
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A Gram-negative, aerobic, non-flagellated and rod-shaped bacterium, strain ASW11-22, was isolated from an intertidal sediment collected from a coastal area of Qingdao, PR China. The strain grew at 15-40 °C (optimum, 37 °C), at pH 6.0-9.

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Two Gram-stain-negative, aerobic, non-motile, and rod-shaped bacterial strains, designated SM1352 and A20, were isolated from intertidal sediments collected from King George Island, Antarctic. They shared 99.8% 16S rRNA gene sequence similarity with each other and had the highest sequence similarity of 98.

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Article Synopsis
  • - The article investigates the viability of using large skin-fat-fascia composite grafts (over 2.0 cm) for repairing partial-thickness nasal defects, challenging traditional recommendations of keeping grafts under 1.5 cm due to higher metabolic needs.
  • - Thirteen patients underwent nasal reconstruction with these larger grafts from October 2017 to December 2019, and all cases showed successful survival of the grafts without complications, evaluated over a follow-up period of 3 to 14 months.
  • - The findings indicate that using a larger postauricular composite graft is an effective method for nasal reconstruction, providing good aesthetic results without extra facial scarring, facial asymmetry, or the need for further revision surgery
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Objective: This study aims to evaluate the clinical effect of modified maximal levator palpebrae superioris shortening method for severe congenital ptosis.

Methods: A retrospective case series was performed including 66 eyes from 62 patients who underwent modified maximal levator palpebrae superioris shortening surgery to treat severe congenital ptosis between February 2015 and November 2018. Preoperative and postoperative margin reflex distance 1 and levator muscle function were recorded.

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Objective: This study aims to evaluate the clinical effect of mini-incision double eyelidplasty in single eye for correcting congenital upper eyelid crease asymmetry.

Methods: Mini-incision double eyelidplasty was performed on 24 patients in single eye to treat congenital upper eyelid crease asymmetry between May 2016 and September 2018. The postoperative surgical results were classified as "excellent," "fair," and "poor.

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Objective: This study was performed to evaluate the clinical effect of skin redraping on lower eyelid epiblepharon accompanied by a medial epicanthal fold.

Methods: This retrospective case series involved 572 eyes of 286 patients who underwent skin redraping surgery to treat lower eyelid epiblepharon accompanied by a medial epicanthal fold from January 2015 to May 2019. The postoperative surgical results were classified as "good", "fair" and "poor".

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A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970, was isolated from a seawater sample collected from the Mariana Trench. Strain SM1970 grew at 15-37 C and with 1-5% (w/v) NaCl. It hydrolyzed colloidal chitin, agar and casein but did not reduce nitrate to nitrite.

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Two Gram-stain-negative bacterial strains, SM1969 and SM1979, were isolated from coastal surface seawater of Qingdao, China. They were taxonomically characterized by the phylogenetic, genomic, chemotaxonomic and phenotypic analyses. The two strains shared 97.

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Two Gram-stain-negative, aerobic, rod-shaped bacteria, polar flagellated, designated strains SM2066 and SM1966, were respectively isolated from the surfaces of and macroalgae collected off the coastal areas of Rongcheng, PR China. Strain SM2066 grew at 8-37 °C and with 0.5-7.

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Keloid is a kind of pathological skin scar with unclear molecular pathology. Circular RNAs (circRNAs) are involved in the occurrence and development of many diseases; however, their relationship with keloid is not well understood. To investigate the involvement of dysregulated circRNAs in keloid.

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Chondrocytes from microtia patients are a valuable cell source for the tissue-engineering of auricles. However, dedifferentiation of microtia chondrocytes remains an obstacle for clinical translation. Strategies, such as three-dimensional (3D) culture systems, and the use of chondrogenic growth factors, have successfully induced redifferentiation of dedifferentiated chondrocytes from healthy individuals.

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A Gram-stain-negative, aerobic, non-flagellated and rod- or ovoid-shaped bacterium, designated as strain S4J41, was isolated from Antarctic intertidal sediment. The isolate grew at 0-37 °C and with 0.5-10 % (w/v) NaCl.

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