Zhongguo Shi Yan Xue Ye Xue Za Zhi
December 2018
Objective: To detect the expression of JAK2/STAT3 mRNA in peripheral blood T cells from the patients with chronic idiopathic thrombocytopenic purpura(CITP), and to explore the relationship between JAK2/STAT3 mRNA and CITP.
Methods: CITP group and healthy control group were set in this study, The JAK2/STAT3 mRNA expression level in peropheral blood T cells of 2 groups was detected with the RT-PCR and agarose gel electrophoresis.
Results: JAK2 mRNA expression level in CITP group was significantly higher than that in control group(P<0.
Zhonghua Zhong Liu Za Zhi
June 2012
Objective: To explore the role of autophagy in quercetin (Que)-induced apoptosis in human bladder carcinoma BIU-87 cells in vitro.
Methods: To determine the proliferative inhibition by MTT colorimetric assay after treating BIU-87 cells with quercetin at various concentrations. To identify autophagy and apoptosis in the BIU-87 cells after Que treatment by monodansylcadaverin (MDC) and Hoechst 33258 fluorescent staining, respectively.