Study on surgical approaches and electrode implantation of oculomotor nerve and inferior obliquus in beagle dogs.

Objectives: To study the surgical anatomy and approaches of intracranial oculomotor nerve (OMN) and inferior obliquus (IO), and the methods of their electrode implantation in dogs.

Methods: The research was performed on 30 adult beagle dogs at Shanghai Jiaotong University Medical College, Shanghai, China from November 2007 to August 2008. All animals were subjected to a right transfrontotemperal approach to intracranial OMN, a transconjunctival route to IO, and the neuro-stimulating and recording electrode implantation under general anaesthesia.

[Overexpression of S3 ribosomal protein gene is involved in drug resistance in K562/DOX cells].

Objective: To investigate the effect of overexpression of S3 ribosomal protein (S3rp) gene on the resistance of leukemia cell to antitumor drugs.

Methods: Both sense and antisense cDNA recombinants of S3rp gene were constructed with pcDNA3.1 expression vector.

[Identification of multidrug resistance related genes in leukemia by suppression subtractive hybridization].

Objective: To clone and screen genes related to multidrug resistance (MDR) in leukemia.

Methods: Suppression subtractive hybridization (SSH) was performed to profile differentially expressed genes between a MDR leukemia cell line (K562/DOX, as tester) and its parent cell line (K562, as driver). Reverse Northern dot blot was carried out to further screen the subtracted cDNA library.