functional validation of anti-CD19 chimeric antigen receptor T cells expressing lysine-specific demethylase 1 short hairpin RNA for the treatment of diffuse large B cell lymphoma.

Background: Chimeric antigen receptor T (CAR-T) cell therapy is more effective in relapsed or refractory diffuse large B cell lymphoma (DLBCL) than other therapies, but a high proportion of patients relapse after CAR-T cell therapy owing to antigen escape, limited persistence of CAR-T cells, and immunosuppression in the tumor microenvironment. CAR-T cell exhaustion is a major cause of relapse. Epigenetic modifications can regulate T cell activation, maturation and depletion; they can be applied to reduce T cell depletion, improve infiltration, and promote memory phenotype formation to reduce relapse after CAR-T cell therapy.

The Mediating Role of Avoidant Personality Disorder Features in the Relationship Between Stressful Life Events and Depression Among First-Year University Students.

Purpose: Stressful life events are risk factors of depression. To explore whether the avoidant personality disorder (AVPD) features play a mediating role between stressful life events (SLEs) and depression among Chinese first-year university students.

Methods: The cross-sectional study was conducted from April to October 2018.

The natural product micheliolide promotes the nuclear translocation of GAPDH via binding to Cys247 and induces glioblastoma cell death in combination with temozolomide.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is significantly upregulated in glioblastoma (GBM) and plays a crucial role in cell apoptosis and drug resistance. Micheliolide (MCL) is a natural product with a variety of antitumour activities, and the fumarate salt form of dimethylamino MCL (DMAMCL; commercial name ACT001) has been tested in clinical trials for recurrent GBM; this compound suppresses the proliferation of GBM cells by rewiring aerobic glycolysis. Herein, we demonstrated that MCL directly targets GAPDH through covalent binding to the cysteine 247 (Cys247) residue.

Establishment and Evaluation of Atmospheric Water Vapor Inversion Model Without Meteorological Parameters Based on Machine Learning.

Precipitable water vapor (PWV) is an important indicator to characterize the spatial and temporal variability of water vapor. A high spatial and temporal resolution of atmospheric precipitable water can be obtained using ground-based GNSS, but its inversion accuracy is usually limited by the weighted mean temperature, Tm. For this reason, based on the data of 17 ground-based GNSS stations and water vapor reanalysis products over 2 years in the Hong Kong region, a new model for water vapor inversion without the Tm parameter is established by deep learning in this paper, the research results showed that, compared with the PWV information calculated by the traditional model using Tm parameter, the accuracy of the PWV retrieved by the new model proposed in this paper is higher, and its accuracy index parameters BIAS, MAE, and RMSE are improved by 38% on average.

GPS Phase Integer Ambiguity Resolution Based on Eliminating Coordinate Parameters and Ant Colony Algorithm.

Correctly fixing the integer ambiguity of GNSS is the key to realizing the application of GNSS high-precision positioning. When solving the float solution of ambiguity based on the double-difference model epoch by epoch, the common method for resolving the integer ambiguity needs to solve the coordinate parameter information, due to the influence of limited GNSS phase data observations. This type of method will lead to an increase in the ill-posedness of the double-difference solution equation, so that the fixed success rate of the integer ambiguity is not high.

Ptosis Correction Through Anterior Displacement of Levator Aponeurosis Within the Corneal Region.

Background: Upper eyelid ptosis is a common aesthetic concern among Asian patients, resulting in a tired and drowsy appearance that affects their attractiveness. The levator advancement technique is widely used for ptosis correction; however, achieving precise results remains challenging.

Objectives: This study introduces a modified approach to improve the accuracy of levator aponeurosis advancement by focusing on precise measurements and anterior displacement within a defined corneal range.

[Relationship between knee meniscus and posterior tibial slope in healthy adults and patients with anteromedial osteoarthritis in Heilongjiang province].

Objective: To measure and analyze the relationships among the posterior tibial slope (PTS), meniscal slope (MS), and meniscus posterior horn thickness (MPHT) of the medial and lateral tibial plateau in healthy people and patients with anteromedial osteoarthritis (AMOA) in Heilongjiang province, so as to provide reference basis for appropriate tibial osteotomy and prosthesis placement angles in knee joint surgeries.

Methods: A retrospective collection of imaging data from knee joint MRI examinations conducted prior to AMOA for various reasons was performed. A total of 103 healthy individuals (healthy group) and 30 AMOA patients (AMOA group) were included.

Advances in Research on Marine Natural Products for Modulating the Inflammatory Microenvironment.

In recent years, marine natural products (MNPs) have emerged as crucial sources of lead compounds for the advancement of anti-inflammatory drugs due to their abundant diversity, complexity, and distinctiveness. Inflammatory microenvironments (IMEs) are pervasive pathological features in the etiology of various chronic diseases, referring to the localized milieu or ecosystem where inflammatory responses occur, and they play a pivotal role in the onset and progression of inflammatory diseases. Uncontrolled IMEs can lead to dysregulation of inflammatory mediators within signaling pathways, thereby exerting detrimental effects on human health and even contributing to the development of inflammatory diseases such as cancer.

Connectional differences between humans and macaques in the MT+ complex.

MT+ is pivotal in the dorsal visual stream, encoding tool-use characteristics such as motion speed and direction. Despite its conservation between humans and monkeys, differences in MT+ spatial location and organization may lead to divergent, yet unexplored, connectivity patterns and functional characteristics. Using diffusion tensor imaging, we examined the structural connectivity of MT+ subregions in macaques and humans.

Comparative clinical efficacy of acupuncture combined with manipulation and other non-pharmacological interventions in the treatment of lumbar disc herniation: a prospective, multi-arm, randomized, open-label, blinded endpoint trial.

Objective: To compare the clinical efficacy and safety of four intervention methods-traditional Chinese manipulation combined with acupuncture, acupuncture alone, manipulation alone, and traction-for the treatment of lumbar disc herniation (LDH).

Methods: A prospective, multi-arm, randomized, parallel-controlled clinical trial was conducted between July 2021 and June 2024. A total of 240 eligible LDH patients were randomized into four groups (60 patients per group) in a 1:1:1:1 ratio: manipulation combined with acupuncture group, manipulation group, acupuncture group, and traction group.

Efficient adsorption behavior of Fe-based ternary magnetic LDHs for naphthalene acetic acid: Role of Fe element.

Naphthalene acetic acid (NAA) is an auxin plant growth regulator (PGR) and widely used to regulate the growth process of plants. As excessive NAA enter the environment, it damages the ecological environment and endangers human life and health. Layered bimetallic hydroxides (LDHs) are widely used for the adsorption of pollutants due to their large surface area and excellent structural properties.

Analysis of the efficacy of lactulose combined with polyethylene glycol in the treatment of functional constipation.

Objectives: This study focuses on analyzing the efficacy of lactulose oral solution combined with Macrogol 4000 Powder in the treatment of functional constipation (FC).

Methods: A total of 125 FC patients were selected, with 60 cases in a control group, who were treated with lactulose oral solution alone, and 65 cases in a research group, who were treated with lactulose oral solution combined with Macrogol 4000 Powder. The two groups were analyzed and compared in terms of efficacy, symptom recovery, Bristol Stool Form Scale (BSFS) and Wexner Constipation Scale (WCS) scores, adverse effects, serum indices, and Patient Assessment of Constipation Quality of Life (PAC-QOL).

Organoid development and applications in gynecological cancers: the new stage of tumor treatment.

Gynecologic cancers (GCs), including cervical cancer (CC), ovarian cancer (OC), endometrial cancer (EC), as well as vulvar and vaginal cancers, represent major health threats to women, with increasing incidence rates observed globally. Conventional treatments, such as surgery, radiation therapy, and chemotherapy, are often hindered by challenges such as drug resistance and recurrence, contributing to high mortality rates. Organoid technology has emerged as a transformative tool in cancer research, offering in vitro models that closely replicate the tumor cell architecture and heterogeneity of primary cancers.

Melanoma bone metastasis-induced osteocyte ferroptosis via the HIF1α-HMOX1 axis.

Osteocytes are the main cells in mineralized bone tissue. Elevated osteocyte apoptosis has been observed in lytic bone lesions of patients with multiple myeloma. However, their precise contribution to bone metastasis remains unclear.

Creatine monohydrate administration delayed muscle glycolysis of antemortem-stressed broilers by enhancing muscle energy status, increasing antioxidant capacity and regulating muscle metabolite profiles.

Preslaughter stress induced a negative energy balance of broilers, resulted in an accelerated glycolysis and finally led to an inferior meat quality. The present study aimed to investigate the effects of creatine monohydrate (CMH) supplementation on muscle energy storage, antioxidant capacity, the glycolysis of postmortem muscle and the metabolite profiles in muscle of broilers subjected to preslaughter transport. Two hundred and forty broilers were chosen and randomly allocated into three treatments (group A, group B and group C), comprising 8 replicates (10 broilers each replicate).

Comparative Study on Growth Characteristics and Early Selection Efficiency of Hybrid Offspring of 'DD-109' and in Liaoning, China.

Poplar is an important tree species for timber supply and ecological protection in northern China. Cultivating and selecting high-quality varieties and germplasm resources suitable for cultivation are key factors in enhancing the quality and productivity of poplar plantations in the arid and semi-arid northern regions with shorter growing seasons. This study conducted a field cultivation experiment on 10 progeny clones from the direct cross (D × M) of imported 'DD-109' with and 7 progeny clones from the reciprocal cross (M × D) using one-year-old rooted cuttings planted at a 4 m × 8 m spacing.

RSL3 induces ferroptosis by activating the NF-κB signalling pathway to enhance the chemosensitivity of triple-negative breast cancer cells to paclitaxel.

Chemotherapy resistance in triple-negative breast cancer (TNBC) leads to poor therapeutic effects and a poor prognosis. Given that paclitaxel-based chemotherapy is the main treatment method for TNBC, enhancing its chemosensitivity has been a research focus. Induced ferroptosis of tumour cells has been proven to increase chemosensitivity, but its ability to sensitize TNBC cells to paclitaxel (PTX) is unknown.

Bone marrow transplantation reverses metabolic alterations in multiple sulfatase deficiency: a case series.

Background: Multiple sulfatase deficiency (MSD) is an exceptionally rare neurodegenerative disorder due to the absence or deficiency of 17 known cellular sulfatases. The activation of all these cellular sulfatases is dependent on the presence of the formylglycine-generating enzyme, which is encoded by the SUMF1 gene. Disease-causing homozygous or compound heterozygous variants in SUMF1 result in MSD.

Untargeted metabolomics analysis as a potential screening tool for 3-methylglutaconic aciduria syndromes.

The 3-methylglutaconic aciduria (3-MGA-uria) syndromes comprise a heterogeneous group of inborn errors of metabolism defined biochemically by detectable elevation of 3-methylglutaconic acid (3-MGA) in the urine. In type 1 (or primary) 3-MGA-uria, distal defects in the leucine catabolism pathway directly cause this elevation. Secondary 3-MGA-uria syndromes, however, are unrelated to leucine metabolism-specific defects but share a common biochemical phenotype of elevated 3-MGA.

Synthesis of α,β-Unsaturated Carbonyl Compounds via Cu/TEMPO/O Aerobic Catalytic System.

An N,N,N-type Cu(II) complex-catalyzed desaturation method for converting alcohols, ketones, lactones, and lactams to their α,β-unsaturated carbonyl compounds is reported. The dehydrogenation reaction can be conducted with a green terminal oxidant O without requiring strong acid/base or stoichiometric oxidants. The Cu(II) complex/TEMPO/O system uses a non-noble catalyst, and a green terminal oxidant as well as demonstrates high activity and functional group tolerance.

Liquid nitrogen quenching for efficient Bifunctional electrocatalysts in water Splitting: Achieving four key objectives in one step.

Herein, a novel liquid nitrogen quenching treatment is proposed to achieve multifaceted modulation involving morphological modulation, lattice tensile strain modulation, metal active centre coordination reconstruction and grain boundary construction within a series of intermetallic compounds modified on a carbon substrate (CoFe-550/C, CoNi-550/C and FeNi-550/C, where 550 refers to liquid nitrogen quenching temperature and C refers to the carbon substrate). Noteworthily, the optimising intermediate absorption/desorption process is achieved by multifaceted modulation. Consequently, CoFe-550/C, CoNi-550/C and FeNi-550/C demonstrate considerable overpotential for hydrogen evolution reaction (59.

Pathogenicity of tick-derived lymphocytic choriomeningitis virus in BALB/c mice.

Background: Lymphocytic choriomeningitis virus (LCMV) is a zoonotic pathogen primarily transmitted by rodents. Recently, LCMV has been detected in ticks from northeastern China; however, the pathogenicity of this virus in murine models remains to be elucidated.

Results: Here, we examined the tick-derived LCMV strain JX14 by inoculating BALB/c mice with 3.

Targeting cryptic allosteric sites of G protein-coupled receptors as a novel strategy for biased drug discovery.

G protein-coupled receptors (GPCRs) represent the largest family of membrane receptors and are highly effective targets for therapeutic drugs. GPCRs couple different downstream effectors, including G proteins (such as Gi/o, Gs, G12, and Gq) and β-arrestins (such as β-arrestin 1 and β-arrestin 2) to mediate diverse cellular and physiological responses. Biased signaling allows for the specific activation of certain pathways from the full range of receptors' signaling capabilities.

Learnable color space conversion and fusion for stain normalization in pathology images.

Variations in hue and contrast are common in H&E-stained pathology images due to differences in slide preparation across various institutions. Such stain variations, while not affecting pathologists much in diagnosing the biopsy, pose significant challenges for computer-assisted diagnostic systems, leading to potential underdiagnosis or misdiagnosis, especially when stain differentiation introduces substantial heterogeneity across datasets from different sources. Traditional stain normalization methods, aimed at mitigating these issues, often require labor-intensive selection of appropriate templates, limiting their practicality and automation.

IL-37 attenuated HPV induced inflammation of oral epithelial cells via inhibiting PI3K/AKT/mTOR.

Human papillomavirus (HPV) is the most prevalent sexually transmitted infection globally, with significant implications for various anogenital cancers, such as vulval, vaginal, anal, penile, head and neck cancers. HPV infections have been linked to the induction of inflammation. In contrast, Interleukin-37 (IL-37) is recognized as an anti-inflammatory cytokine.