Clinical use of tumor biomarkers in prediction for prognosis and chemotherapeutic effect in esophageal squamous cell carcinoma.

Background: Growing evidence has indicated that tumor biomarkers, including cytokeratin 19 fragment antigen 21-1 (Cyfra21-1), carbohydrate antigen 19-9 (CA19-9), carbohydrate antigen 72-4 (CA72-4), carcinoembryonic antigen (CEA) and squamous cell carcinoma antigen (SCC-Ag) were reported to be commonly used in diagnosis and prognosis in esophageal squamous cell carcinoma (ESCC). However, which is the best marker for predicting prognosis remains unknown. Few papers focused on the relationship between tumor biomarkers and postoperative treatment in ESCC.

Utilization of glycerol and crude glycerol for polysaccharide production by an endophytic fungus CGMCC 6882.

Crude glycerol is becoming a financial and environmental liability due to its surplus production from biodiesel industry, and its utilization as a fermentation feedstock for value-added chemicals production has been widely studied. In present work, the capacity of an endophytic fungus, CGMCC 6882, using glycerol and crude glycerol for polysaccharide production was investigated. Results showed that the polysaccharide titers from glucose and glycerol were 1.

Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.

Introduction: Alginate/gelatin hydrogel (Alg-Gel) scaffold has been applied in tissue engineering, but the research on its application in dental tissues regeneration is still lacking. We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).

Methods: hDPSCs were cultured in both Alg-Gel and 3D-printed Alg-Gel scaffolds.

Correlates of HIV-infection among men who have sex with men: results from a community-based, cross-sectional study in Tianjin, China.

China has 747,000 confirmed HIV/AIDS cases and sexual transmission between men who have sex with men (MSM) is the most prevalent mode of transmission. Our study aims to (a) examine HIV prevalence and behavioral risk factors for HIV infection among 1900 MSM recruited from a community-based organization in Tianjin, China, and (b) describe HIV treatment outcomes for those diagnosed with HIV. We used multivariable logistic regression to identify which socio-demographics and behaviors were associated with HIV infection.

Stathmin regulates the proliferation and odontoblastic/osteogenic differentiation of human dental pulp stem cells through Wnt/β-catenin signaling pathway.

Odontoblastic/osteogenic differentiation of human dental pulp stem cells (hDPSCs) is a key factor in tooth and pulp regeneration, but its mechanism still remains unknown. The purpose of this research is to look into the mechanism by which Stathmin affects the proliferation and odontoblastic/osteogenic differentiation of hDPSCs, and whether the Wnt/β- catenin is related to this regulation. First, the Stathmin expression was inhibited by lentiviral vector, after that the transcriptome sequencing technology was used to screen the differentially expressed genes, then we found Wnt5a which related to the regulation of Wnt/β-catenin was regulated.

Tunable hybridization of graphene plasmons and dielectric modes for highly confined light transmit at terahertz wavelength.

We theoretically report a novel graphene-based hybrid plasmonic waveguide (GHPW) by integrating a GaAs micro-tube on a silica spacer that is supported by a graphene-coated substrate. In comprehensive numerical simulations on guiding properties of the GHPW, it was found that the size of hybrid plasmonic mode (TM) can be reduced significantly to ~10(λ/4), in conjunction with long propagation distances up to tens of micrometers by tuning the the waveguide's key structure parameters and graphene's chemical potential. Moreover, crosstalk between two adjacent GHPWs that are placed on the same substrate has been analyzed and ultralow crosstalk can be realized.

Nipah pseudovirus system enables evaluation of vaccines in vitro and in vivo using non-BSL-4 facilities.

Because of its high infectivity in humans and the lack of effective vaccines, Nipah virus is classified as a category C agent and handling has to be performed under biosafety level 4 conditions in non-endemic countries, which has hindered the development of vaccines. Based on a highly efficient pseudovirus production system using a modified HIV backbone vector, a pseudovirus-based mouse model has been developed for evaluating the efficacy of Nipah vaccines in biosafety level 2 facilities. For the first time, the correlates of protection have been identified in a mouse model.

Discovery of 5-Signature Predicting Survival of Patients with Lower-Grade Glioma.

Objective: In the study, we aimed to identify key microRNAs (miRNAs) and clinical factors associated with survival time of lower-grade glioma (LGG) and develop an expression-based miRNA signature to provide survival risk prediction for patients with LGG.

Methods: We obtained miRNA expression profiles and clinical information of patients with LGG from The Cancer Genome Atlas dataset. All 591 miRNAs were modeled using random Forest Survival, Regression, and Classification to construct a random forest model for survival analysis, and feature selection was performed.

[The characteristics of recent HIV-1 infection and associated factors in Tianjin].

The newly reported HIV infected cases was collected, and HIV blood samples were detected to identify recent HIV infection in Tianjin during 2008-2015. Factors associated with HIV-1 infection were analyzed by the univariate and multivariate unconditional logistic regression. The recent HIV-1 infection proportion of homosexuals increased from 37.

Climate background, relative rate, and runoff effect of multiphase water transformation in Qilian Mountains, the third pole region.

Multiphase water transformation has great effects on alpine hydrology, but these effects remain unclear in the third pole region. Taking the Qilian Mountains as an example, the climate background and relative rates of multiphase water transformation were analyzed, and the runoff effect was evaluated based on long-term field observations. There are three climatic aspects driving multiphase water transformation, including lengthening ablation period, accelerative warming after 1990, and larger warming in the cryosphere belt than in the vegetation belt.

Nanosecond pulsed electric fields enhanced chondrogenic potential of mesenchymal stem cells via JNK/CREB-STAT3 signaling pathway.

Background: Nanosecond pulsed electric fields (nsPEFs) can produce more significant biological effects than traditional electric fields and have thus attracted rising attention in developing medical applications based on short pulse duration and high field strength, such as effective cancer therapy. However, little is known about their effects on the differentiation of stem cells. Furthermore, mechanisms of electric fields on chondrogenic differentiation of mesenchymal stem cells (MSCs) remain elusive, and effects of electric fields on cartilage regeneration need to be verified in vivo.

Wide tuning range and high OSNR self-seeded multi-wavelength Brillouin-erbium fiber laser based on a Lyot filter.

A tunable self-seeded multi-wavelength Brillouin-erbium fiber laser (BEFL) based on a Lyot filter is experimentally demonstrated in this paper. The Lyot filter is constructed by a polarization-dependent isolator (PDI), two polarization controllers (PCs), and a 20-cm-length polarization-maintaining fiber. In this proposed structure, the PDI is used as a polarizer.

Effectiveness of Intensive Endoscopic Screening for Esophageal Cancer in China: A Community-Based Study.

Evidence is required to evaluate the effectiveness of population-level endoscopic screening for esophageal cancer (EC). In this study, 5,632 permanent residents aged 25-65 years from 6 villages in Hua County, Henan Province, China, were defined as the screening cohort and were offered intensive endoscopic screening. Residents of all 914 remaining villages in Hua County were included as the control cohort, and age-sex standardization was used to calculate the expected numbers of EC and upper gastrointestinal (GI) tract cancer cases and deaths in the screening cohort.

MiR-181a, a new regulator of TGF-β signaling, can promote cell migration and proliferation in gastric cancer.

Transforming growth factor-beta (TGF-β) signaling pathway plays pivotal roles in various types of cancer. TGF-β receptor 2 (TGFβR2) contains a kinase domain that phosphorylates and activates the downstream of the TGF-β signaling pathway. Our previous microarray analysis revealed marked changes in miR-181a expression in gastric cancers, and the bioinformatics analysis suggested that miR-181a negatively regulated TGFβR2.

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Our previous study showed that knocking down integrin α5 (ITGA5) expression by using a lentiviral vector in human dental pulp stem cells (DPSCs) led to weakening proliferation and migration capacity while enhanced odontogenic differentiation. To seek for possible clinical application, we investigated the effect of the ITGA5 priming synthetic cyclic peptide (SCP; GA-CRRETAWAC-GA) on proliferation, migration, and the odontogenic differentiation of DPSCs. Remarkably, the involved mechanism was explored by isobaric tag for relative and absolute quantitation proteomic technique, and the in vivo effect of ITGA5 was investigated by nude mice subcutaneous transplantation of cell and hydroxyapatite/β-tricalcium phosphate complex.

Exosome circRNA secreted from adipocytes promotes the growth of hepatocellular carcinoma by targeting deubiquitination-related USP7.

Hepatocellular carcinoma (HCC), the major form of liver cancer, has shown increasing incidence and poor prognosis. Adipose tissue is known to function in energy storage and metabolism regulation by the secretion of adipokines. Circular RNAs (circRNAs), a novel type of noncoding RNA, have recently been recognized as key factors in tumor development, but the role of exosome circRNAs derived from adipose tissues has not been defined yet.

[Study on acute HIV-1 infection in men who have sex with men in Tianjin].

To understand the immunological and virological characteristics of HIV-1 infected men who have sex with men (MSM) in the acute phase in Tianjin and evaluate the effects of the fourth generation HIV ELISA and the P24 ELISA for acute HIV-1 infected samples. From October 2015 to October 2016, MSM were recruited through the community-based organizations in Tianjin. All the participants received rapid HIV test, positive samples were confirmed by Western Blot and negative samples underwent pooled nucleic acid testing.

Chaotic Analysis of Hippocampal and Cortical Sleep EEG during Various Vigilance States.

In this paper, we investigate the hippocampal and cortical sleep EEG of adult rats at different sleep stages by employing Lyapunov exponent and third-order cumulant measures to quantify and compare the chaotic and nonlinear behavior of EEG obtained during vigilance states of quiet- waking, slow-wave sleep, and rapid eye movement (REM) sleep. Lyapunov exponent was computed to characterize the EEG for chaos and third-order cumulant was used to measure the deviations from Gaussianity of the signal. Our results show positive Lyapunov exponents for all EEG states indicating a Iow- dimensional chaos for both REM and non-REM system.

Microscopic and Submicroscopic Gradient Variation of Olfactory Systems among Six Sinocyclocheilus Species Living in Different Environments.

The fish genus Sinocyclocheilus contains many different species that inhabit diverse natural environments, such as surface water layer, cave, or intermediate. As a result of these different habitats there are some differences in their sensory systems. Microscopic and submicroscopic structures of olfactory systems in six representative species of Sinocyclocheilus were studied, including one surface-dwelling species (S.

Exosomal miR-27a Derived from Gastric Cancer Cells Regulates the Transformation of Fibroblasts into Cancer-Associated Fibroblasts.

Background/aims: The malignant biological behavior of gastric cancer(GC) is not only determined by cancer cells alone, but also closely regulated by the microenvironment. Fibroblasts represent a large proportion of the components in the tumor microenvironment, and they promote the development of disease. Currently, accumulating evidence suggests that exosomes can function as intercellular transport systems to relay their contents, especially microRNAs(miRNAs).

[Effects of ditch-buried organic matter and irrigation amount after anthesis on the photosynthetic characteristics and yield of maize.].

Managements of organic matter and irrigation after anthesis will increase the capacities of water conservation and supply in maize field, with consequences on photosynthetic performance and yield under water-saving condition. We analyzed the gas exchange parameters and the performance of the photosystem 2 of ear leaves, and yield of maize cultivars Zhengdan 958, under three modes of ditch-buried organic matter (no straw returned: M, wheat straw returned: M, mixtures of cow manure and wheat straw returned: M) before seeding with two irrigation levels after anthesis (normal irrigation: W, water-saving irrigation: W). The results showed that M treatment significantly increased photosynthetic capacity and dry matter accumulation after anthesis compared with M treatment.

Bend-resistant leaky multi-trench fiber with large mode area and single-mode operation.

A novel structure of modified multi-trench fiber (MTF) with characteristics of bend-resistance and large mode-area is proposed. In this structure, each low refractive-index trench of traditional MTF is broken by two gaps up and down. Numerical investigations show that the mode field area of 840 μm2 can be achieved with effective single-mode (SM) operation when the bending radius is 15 cm.

Comparison of the effectiveness of four Budyko-based methods in attributing long-term changes in actual evapotranspiration.

The responses of hydrological processes to climate change and anthropogenic influence have received significant attention over the past few decades. Several Budyko-based methods have been widely used to attribute hydrological variations and identify the extent of variation due to climate change and human activities. However, the accuracy of various methods has rarely been compared.

Expression of APOB, ADFP and FATP1 and their correlation with fat deposition in Yunnan's top six famous chicken breeds.

1. Adipose differentiation related protein (ADFP), fatty acid transport protein 1 (FATP1) and apolipoprotein B (APOB) are suspected to play an important role in determining intramuscular fat and in overall meat quality. 2.