Phosphate Limitation Enhances Heterologous Enzyme Production in : Mechanistic Insights and Universal Applicability.

is one of the commonly used hosts for heterologous enzyme expression, depending on media rich in carbon, nitrogen, and phosphate sources for optimal growth and enzyme production. Interestingly, our investigation of maltotetraose-forming amylase, a key enzyme for efficient maltotetraose synthesis, revealed that phosphate limitation significantly enhances the growth rate and production of heterologous enzymes in recombinant . Under phosphate-limited conditions in a 15 L fermenter, the enzyme activity reached 679.

Bismuth-doped fiber amplifier with controlled gain and extended bandwidth based on tunable gain clamping.

The study of gain clamping in broadband bismuth-doped fiber amplifiers (BDFA) not only helps to solve the gain instability problem due to the variation of the number of optical multiplexing channels but also is an effective way to extend the amplifier's operating bandwidth to improve the communication capacity. In this paper, we illustrate the advantages of linear cavity gain clamping through simulation. Using simulation to guide the experiments, we propose a BDFA with tunable linear-cavity gain clamping and incorporate a variable optical attenuator (VOA) in the linear cavity to enhance the flexibility of gain control.

Clinical efficacy of antibiotic-loaded bone cement and negative pressure wound therapy in multidrug-resistant organisms diabetic foot ulcers: a retrospective analysis.

Objective: The purpose of this study was to investigate the clinical efficacy of antibiotic-loaded bone cement (ALBC) combined with Negative pressure wound therapy (NPWT) aspiration technique in the treatment of multidrug-resistant diabetic foot ulcers (MDRO-DFUs).

Methods: A retrospective analysis of the clinical data of 80 patients with MDROs-DFU who were used Vacuum sealing drainage (VSD) as NPWT excipient and met the inclusion criteria from January 2019 to January 2024 at our hospital. Patients were divided into an experimental group and a control group, with 40 cases in each.

Broadband polarization-maintaining anti-resonant fiber design via swarm intelligence.

In this study, we utilized a discrete point configuration method in conjunction with genetic algorithm (GA) and particle swarm optimization (PSO) to design broadband polarization-maintaining anti-resonant fibers (PM-ARFs). The resulting structure features a confinement loss (CL) below 0.17 dB/m, birefringence of approximately 8.

Efficacy and safety of multi-target tyrosine kinase inhibitor AL2846 combined with gemcitabine in pancreatic cancer.

Pancreatic cancer patients urgently need new treatments, and we explored the efficacy and safety of combination therapy with AL2846 and gemcitabine in pancreatic cancer patients. This was a single-arm, single-center, open-label phase I/IIa study (NCT06278493). The dose-escalation phase was designed to evaluate the maximum tolerated dose (MTD) of AL2846 combined with gemcitabine.

[Analysis of human papillomavirus infection status and related factors among men who have sex with men in Tianjin].

The status quo and related factors of human papillomavirus (HPV) infection in multiple parts of men who have sex with men (MSM) in Tianjin were analyzed. Using the cross-sectional survey method, a total of 600 MSM were recruited in Tianjin from September to December 2023, including 200 (18-26 years old), 300 (27-45 years old), and 100 (≥46 years old) stratificaed by age group. Questionnaire survey was used to collect knowledge and behavioral information related to demography and STD prevention and treatment.

Standardly Trained Peer Volunteer-Led, Social App Recruiting-Based Human Immunodeficiency Virus Testing Strategy Using Rapid Testing Kits (SPARK) Among Men Who Have Sex With Men: A Population-Based Survey Evaluation.

Background: Social app recruiting-based and peer-led testing strategies have been proven effective in increasing human immunodeficiency virus (HIV) testing among men who have sex with men (MSM), though their combination remains underevaluated. We aimed to assess the efficiency of a combined strategy named "standardly trained peer volunteer-led, social app recruiting-based HIV testing strategy using rapid testing kits" (SPARK).

Methods: Between March 2020 and December 2021, 177 trained peer volunteers tested 7256 eligible MSM testers.

PANoptosis-related genes: Molecular insights into immune dysregulation in ulcerative colitis.

Background And Aim: Ulcerative colitis (UC) is a chronic inflammatory disease driven by immune dysregulation. PANoptosis, a novel form of programmed cell death, has been implicated in inflammatory diseases, but its specific role in UC remains unclear. This study aimed to identify PANoptosis-related genes (PRGs) that may contribute to immune dysregulation in UC.

Analysis of multi-site HPV infection and vaccination willingness among men who have sex with men in Tianjin, China.

Background: Men who have sex with men (MSM) are vulnerable to HPV infection. This study aims to explore the HPV infection status at different sites among HIV-positive MSM, HIV-negative MSM, and men who have sex with women (MSW), and to investigate their willingness to receive HPV vaccination.

Methods: From September 2023 to April 2024, three groups were recruited in Tianjin, China.

Comprehensive sequencing of the genome and transcriptome of the Xishuangbanna game fowl.

The Xishuangbanna game fowl, a local Chinese chicken breed renowned for its athleticism, is facing extinction. Despite its significance, research on its genome assembly and resequencing remains sparse, limiting our understanding of its genetic structure and function. In this study, we assembled a chromosome-level genome using second-generation sequencing and high-throughput chromosome conformation capture (Hi-C), along with transcriptome sequencing.

PTOV1 facilitates colorectal cancer cell proliferation through activating AKT1 signaling pathway.

Background: Colorectal cancer is a predominant contributor to global cancer-related morbidity and mortality. The oncogene PTOV1 has been linked to various human malignancies, yet its specific role in CRC pathogenesis requires further elucidation.

Methods: Our study used a comprehensive array of authoritative bioinformatics tools, such as TIMER, UCSC Xena, GEO, Human Protein Atlas, UALCAN, CIBERSORTx and others which used to investigate the complex effects of PTOV1 on gene expression profiles, diagnostic and prognostic biomarkers, tumor immunology, signaling pathways, epigenetic alterations, and genetic mutations.

Revealing the Viable Microbial Community of Biofilm in a Sewage Treatment System Using Propidium Monoazide Combined with Real-Time PCR and Metagenomics.

Microbial community composition, function, and viability are important for biofilm-based sewage treatment technologies. Most studies of microbial communities mainly rely on the total deoxyribonucleic acid (DNA) extracted from the biofilm. However, nucleotide materials released from dead microorganisms may interfere with the analysis of viable microorganisms and their metabolic potential.

Functionally improved mesenchymal stem cells via nanosecond pulsed electric fields for better treatment of osteoarthritis.

Background: Numerous approaches have been utilized to optimize mesenchymal stem cells (MSCs) performance in treating osteoarthritis (OA), however, the constrained diminished activity and chondrogenic differentiation capacity impede their therapeutic efficacy. Previous investigations have successfully shown that pretreatment with nanosecond pulsed electric fields (nsPEFs) significantly enhances the chondrogenic differentiation of MSCs. Therefore, this study aims to explore nsPEFs as a strategy to improve OA therapy by enhancing MSCs' activity and chondrogenic differentiation and also investigate its potential mechanism.

Changes in hemicellulose metabolism in banana peel during fruit development and ripening.

Hemicellulose is key in determining the fate of plant cell wall in almost all growth and developmental stages. Nevertheless, there is limited knowledge regarding its involvement in the development and ripening of banana fruit. This study investigated changes in the temporal-spatial distribution of various hemicellulose components, hemicellulose content, activities of the main hydrolysis enzymes, and transcription level of the main hemicellulose-related gene families in banana peels.

Response of soil microbial community structure and function to the sewage leakage: A case study of a 25-year-old cesspool.

Providing many millions of rural households with decentralized sanitation facilities remains challenging. In undeveloped areas, cesspools have still been widely used due to technologically simple and low-cost. However, the influence of cesspools on the surrounding soil remains unclear.

A Mendelian randomization study: Years of education and nonalcoholic fatty liver disease.

Though years of education have been connected to nonalcoholic fatty liver disease (NAFLD), the exact mechanism underlying this linkage is still unknown. To investigate the causal association between years of education and NAFLD, we will use a 2-sample Mendelian randomization (MR) technique. : Genome-wide association studies data on years of education (n = 766,345) and genome-wide association studies data on nonaffiliated mental illness (n = 778,614) were screened for genetic variations as instrumental variables in the Mr-Base database.

Effects of Micro-Topography and Vegetation on Soil Moisture on Fixed Sand Dunes in Tengger Desert, China.

Soil moisture is a key factor in arid ecosystems, with local variations influenced by topography and vegetation. Understanding this relationship is crucial for combating desertification. Employing ANOVA, Mean Decrease Accuracy (MDA) analysis from random forest modeling and Structural Equation Modeling (SEM), this study investigates the distribution of soil moisture and its associations with topographic and vegetative factors across four micro-geomorphic units in the Tengger Desert, China.