Publications by authors named "Nina Mejlhede"
Coinfection with multiple human papilloma virus (HPV) types is common in cervical HPV infection. To evaluate if infections with different HPV types occur independently, we examined 3558 women above 15 years of age suspected of cervical HPV infection. Among them, 1842 (52%) women were HPV negative and 1716 (48%) were HPV positive as analysed by a PCR-based commercial microarray assay for mucosal types.
View Article and Find Full Text PDFGenital human papillomavirus infection (HPV) is common and usually harmless. However, chronic cervical infection with high-risk HPV types can cause cell changes that may eventually lead to cancer. To determine the frequency of individual HPV types among mixed infections, we examined the type distribution among cervical specimens from more than 1000 Danish women.
View Article and Find Full Text PDFThe discovery of programmed -1 frameshifting at the hexanucleotide shift site CGA_AAG, in addition to the classical X_XXY_YYZ heptanucleotide shift sequences, prompted a search for instances among eubacterial insertion sequence elements. IS1222 has a CGA_AAG shift site. A genetic analysis revealed that frameshifting at this site is required for transposition.
View Article and Find Full Text PDFProgrammed -1 ribosomal frameshifting, involving tRNA re-pairing from an AAG codon to an AAA codon, has been reported to occur at the sequences CGA AAG and CAA AAG. In this study, using the recoding region of insertion sequence IS3, we have investigated the influence on frameshifting in Escherichia coli of the first codon of this type of motif by changing it to all other NNA codons. Two classes of NNA codons were distinguished, depending on whether they favor or limit frameshifting.
View Article and Find Full Text PDFArticle Synopsis
- The protein lysyl-tRNA synthetase (LysRS) has two forms: class I and class II, with Borrelia burgdorferi having the class I type while its hosts have class II.
- Researchers cloned and sequenced the lysK gene from several Borrelia species to explore its potential as a diagnostic target for Lyme disease.
- Using these sequences, a primer set was developed for detecting and genotyping different Borrelia species in one PCR test, ensuring specificity by avoiding cross-reactivity with other bacteria.