A systematic review protocol for quantifying bycatch of critically endangered leatherback sea turtles within the Pacific Ocean basin.

Background: The Pacific Ocean supports two leatherback sea turtle populations, each of which is Critically Endangered primarily as a result of ongoing incidental bycatch within small-scale and industrial fisheries. Conservation planning has included population viability analysis (PVA), which depends on accurate data on mortality and morbidity (sublethal effects) rates to yield realistic results that can inform management decision-making. Existing leatherback PVAs are based on best available data, however, estimates of mortality and morbidity rates are heavily influenced by estimates of bycatch.

Multidimensional scaling for animal traits in the context of dynamic energy budget theory.

The method of multidimensional scaling (MDS) has long existed, but could only recently be applied to animal traits in the context of dynamic energy budget (DEB) theory. The application became possible because of the following: (i) the Add-my-Pet (AmP) collection of DEB parameters and traits (approximately 280) recently reached 3000 animal species with 45000 data sets of measurements; (ii) we found a natural distance measure for species based on their traits as a side result of our research on parameter estimation in DEB context; and (iii) we developed plotting code for visualization that allows labelling of taxonomic relationships. Traits, here defined as DEB parameters or any function of these parameters, have different dimensions, which hamper application of many popular distance measures since they (implicitly) assume that all traits have the same dimensions.

Energetic basis for bird ontogeny and egg-laying applied to the bobwhite quail.

Birds build up their reproductive system and undergo major tissue remodeling for each reproductive season. Energetic specifics of this process are still not completely clear, despite the increasing interest. We focused on the bobwhite quail - one of the most intensely studied species due to commercial and conservation interest - to elucidate the energy fluxes associated with reproduction, including the fate of the extra assimilates ingested prior to and during reproduction.

Investigating the Ability of Growth Models to Predict In Situ spp. Abundances.

spp. have an important role in biogeochemical cycles; some species are disease agents for aquatic animals and/or humans. Predicting population dynamics of spp.

The comparative energetics of the turtles and crocodiles.

The Add-my-Pet collection of data on energetics and Dynamic Energy Budget parameters currently contains 92 species of turtles and 23 species of crocodiles. We discuss patterns of eco-physiological traits of turtles and crocodiles, as functions of parameter values, and compare them with other taxa. Turtles and crocodiles accurately match the general rule that the life-time cumulated neonate mass production equals ultimate weight.

Physiological performance of native and invasive crayfish species in a changing environment: insights from Dynamic Energy Budget models.

Crayfish are keystone species important for maintaining healthy freshwater ecosystems. Crayfish species native to Europe, such as and , are facing decline and are increasingly endangered by changing climate and invasions of non-native crayfish, such as and . The success of these invasions largely depends on differences in ontogeny between the native species and the invaders and how changes in the environment will affect the ontogeny.

Quantifying impacts of plastic debris on marine wildlife identifies ecological breakpoints.

Quantifying sublethal effects of plastics ingestion on marine wildlife is difficult, but key to understanding the ontogeny and population dynamics of affected species. We developed a method that overcomes the difficulties by modelling individual ontogeny under reduced energy intake and expenditure caused by debris ingestion. The predicted ontogeny is combined with a population dynamics model to identify ecological breakpoints: cessation of reproduction or negative population growth.

Predicting perceived level of disturbance of visitors due to crowding in protected areas.

Managing the disturbance of visitors due to crowding is an important management task in protected areas with high use levels. To achieve this, managers need to know how the use level affects the perceived disturbance due to crowding. Here we present a method to predict the level of disturbance as a function of use level measured by number of visitors.

The universality and the future prospects of physiological energetics: Reply to comments on "Physics of metabolic organization".

In response to the comments on review "Physics of metabolic organization", we discuss the universality and the future prospects of physiological energetics. The topics range from the role of entropy in modeling living organisms to the apparent ubiquity of the von Bertalanffy curve, and the potential applications of the theory in yet unexplored domains. Tradeoffs in outreach to non-specialists are also briefly considered.

Inferring physiological energetics of loggerhead turtle (Caretta caretta) from existing data using a general metabolic theory.

Loggerhead turtle is an endangered sea turtle species with a migratory lifestyle and worldwide distribution, experiencing markedly different habitats throughout its lifetime. Environmental conditions, especially food availability and temperature, constrain the acquisition and the use of available energy, thus affecting physiological processes such as growth, maturation, and reproduction. These physiological processes at the population level determine survival, fecundity, and ultimately the population growth rate-a key indicator of the success of conservation efforts.

Physics of metabolic organization.

We review the most comprehensive metabolic theory of life existing to date. A special focus is given to the thermodynamic roots of this theory and to implications that the laws of physics-such as the conservation of mass and energy-have on all life. Both the theoretical foundations and biological applications are covered.

Size Scaling in Western North Atlantic Loggerhead Turtles Permits Extrapolation between Regions, but Not Life Stages.

Introduction: Sea turtles face threats globally and are protected by national and international laws. Allometry and scaling models greatly aid sea turtle conservation and research, and help to better understand the biology of sea turtles. Scaling, however, may differ between regions and/or life stages.

Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro.

Aim: To establish an organotypic in vitro model of limb bud development to verify whether epigenetic drug and teratogen 5-azacytidine (5azaC) has an effect on limb buds independent of its effects on the placenta.

Methods: Fischer strain rat fore- and hindlimb buds were microsurgically isolated from 13 days old embryos and cultivated in vitro for two weeks at the air-liquid interface in Eagle's minimum essential medium (MEM) with 50% rat serum. 30 μmol of 5azaC was added to the fresh medium.