Protein Synthesis Determined from Non-Radioactive Phenylalanine Incorporated by Antarctic Fish.

Direct measurements of temperature-dependent weight gains are experimentally challenging and time-consuming in long-lived/slow-growing organisms such as Antarctic fish. Here, we reassess methodology to quantify the in vivo protein synthesis rate from amino acids, as a key component of growth. We tested whether it is possible to avoid hazardous radioactive materials and whether the analytical pathway chosen is robust against analytical errors.

Long-term effects of elevated pCO levels on the expression of Chaoborus-induced defences in Daphnia pulex.

Increased carbon dioxide from fossil fuel combustion results in an enrichment of CO in the global carbon cycle. Recent evidence indicates that rising atmospheric CO impacts the partial pressure of carbon dioxide (pCO) in freshwaters. This affects freshwater biota by disrupting chemical communication between predator and prey.

Elevated pCO affects behavioural patterns and mechano-sensation in predatory phantom midge larvae Chaoborus obscuripes.

Aquatic acidification is a major consequence of fossil fuel combustion. In marine ecosystems it was shown, that increasing pCO levels significantly affect behavioural and sensory capacities in a diversity of species. This can result in altered predator and prey interactions and thereby change community structures.

Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia.

Background: Diapause is a form of dormancy that is genetically predetermined to allow animals to overcome harsh environmental conditions. It is induced by predictive environmental cues bringing cellular activity levels into a state of suspended animation. Entering diapause requires organismal, molecular and cellular adaptation to severely reduced energy flows.