Publications by authors named "Nina Boskovic"
Article Synopsis
- This study focuses on embryonic genome activation (EGA) in bovine embryonic development, highlighting the need to understand the role of PRD-like (PRDL) transcription factors that are crucial for this process but have evolved differently across species.
- Molecular cloning was performed on several PRDL TFs in bovine oocytes and IVF preimplantation embryos, confirming their expression and revealing breed-related genetic variations through the identification of numerous single nucleotide variants (SNVs).
- The research demonstrated that overexpressing the TPRX3 gene in bovine fibroblasts activates not only protein-coding genes but also small noncoding RNAs, supporting the notion of shared gene sets in early bovine
Purpose: Ovarian tissue cryopreservation is vital for fertility preservation, yet its effect on ovarian tissue follicle survival and transcriptomic signature requires further investigation. This study delves into the effects of vitrification on tissue morphology, function, and transcriptomic changes, helping to find possibilities for vitrification protocol improvements.
Methods: Ovarian cortex from 19 bovine animals were used to conduct pre- and post-vitrification culture followed by histological assessment, immunohistochemistry, and TUNEL assay.
Here, we present a modification of single-cell tagged reverse transcription protocol to study gene expression on a single-cell level or with limited RNA input. We describe different enzymes for reverse transcription and cDNA amplification, modified lysis buffer, and additional clean-up steps before cDNA amplification. We also detail an optimized single-cell RNA sequencing method for handpicked single cells, or tens to hundreds of cells, as input material to study mammalian preimplantation development.
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