Detection of apoptotic and live pre-osteoblast cell line using impedance-based biosensors with variable electrode design.

Electrical impedance-based sensing of cell activity has become a powerful analytical tool that allows the monitoring of several relevant biological processes associated with cell evolution and morphology. In these types of biosensors, the electrode design has a direct impact on the sensitivity because it defines the capability of the biosensor to measure small changes in the impedance resulting from cell activities. Herein, impedance-based biosensors arrays with several configurations were successfully developed and used to study the impact of the electrode layout on the dynamics of cultured pre-osteoblast cells.

Biofunctionalized two-dimensional TiC MXenes for ultrasensitive detection of cancer biomarker.

In this work, ultrathin TiC-MXene nanosheets were synthesized by minimally intensive layer delamination methods, and uniformly functionalized with aminosilane (f-TiC-MXene) to provide a covalent binding for the immobilized bio-receptor (anti-CEA) for label free, ultrasensitive detection of cancer biomarker (carcinoembryonic antigen, CEA). The effect of different redox probes on the electrochemical behavior of f-TiC-MXene was investigated and found that hexaammineruthenium ([Ru(NH)]) is the preferable redox probe for biosensing. The fabricated biofunctionalized TiC-MXene exhibits a linear detection range of 0.