This study investigates the combined toxicity of microplastics (MPs) and florfenicol (FLO) on biotransformation enzymes and oxidative stress biomarkers in the liver and kidney of yellowfin seabream (Acanthopagrus latus). Fish were fed 15 mg kg of FLO and 100 or 500 mg kg of MPs for 10 days. Biomarkers, including ethoxyresorufin-O-deethylase, glutathione-S-transferase, superoxide dismutase, catalase, glutathione peroxidase, malondialdehyde (MDA), and protein carbonylation (PC), were measured in both organs at 1, 7, and 14 days post-exposure.
View Article and Find Full Text PDFThe aquaculture industry frequently employs various slaughter methods that subject fish to inhumane conditions, resulting in significant suffering. This study examined the effects of electrical method on the welfare of farmed fish () compared to air asphyxiation. Fish captured with electricity exhibited calm behavior until death, in contrast to fish treated with air asphyxiation, which exhibited violent responses within 4 minutes of capture.
View Article and Find Full Text PDFPlastic waste and micro/nanoplastic particles pose a significant global environmental challenge, along with concerns surrounding certain pesticides' impact on aquatic organisms. This study investigated the effects of microplastic particles (MPPs) and cypermethrin (CYP) on crayfish, focusing on biochemical indices, lipid peroxidation, oxidative stress, hematological changes, and histopathological damage. After determining the LC-96 h value (4.
View Article and Find Full Text PDFConcerns have been raised about the possible environmental effects of methyl tert-butyl ether (MTBE), which is widely used as a gasoline additive. This research aimed to look at the consequences of MTBE contamination on rainbow trout (Oncorhynchus mykiss), emphasizing oxidative stress, genotoxicity, and histopathological damage. After determining the LC-96 h value, the effects of sub-lethal doses of MTBE (0 (control), 90, 180, and 450 ppm) on rainbow trout were investigated.
View Article and Find Full Text PDFSwan mussels (Anodonta cygnea) have been suggested as suitable bioindicators for the presence of pollutants in the environment. Application of the physiological and behavioral markers in these sessile species can be beneficial for environmental monitoring. The present study aimed to investigate the relationship between the behavioral disorders of movement and siphoning associated with the inhibition of tissue Acetylcholinesterase (AChE).
View Article and Find Full Text PDFThe toxicological effects of three commonly used pesticides in Iran on the fries of Caspian kutum (, Kamensky, 1901) were investigated through determining 50.00% lethal concentration (LC) 96hr and cholinesterase (ChE) inhibition. The LC 96-hr of carbaryl, glyphosate, and malathion were equal to 11.
View Article and Find Full Text PDFThis study evaluated changes in cutaneous mucosal immunity (total protein (TP) and immunoglobulin (TIg), lysozyme, protease, esterase, and alkaline phosphatase (ALP)) and some immune-related genes expression (tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-8, hepcidin-like antimicrobial peptides (HAMP), and immunoglobulin M (IgM)) in the intestine of rainbow trout (Oncorhynchus mykiss) orally-administrated florfenicol (FFC) and/or olive leaf extract (OLE), experimentally infected with Streptococcus iniae. The juvenile fish (55 ± 7.6 g) were divided into different groups according to the use of added OLE (80 g kg food), the presence/absence of FFC (15 mg kg body weight for 10 consecutive days), and the streptococcal infectivity (2.
View Article and Find Full Text PDFEffects of sub-lethal concentrations (0 (control), 0.009, 0.014, and 0.
View Article and Find Full Text PDFThe purpose of the present work was to determine whether florfenicol (FFC) as the prominent broad-spectrum antibiotic could affect serum biochemical and immunological parameters, as well as immune-related genes expression in rainbow trout (55 ± 7.6 g) challenged with the Lactococcus garvieae and Streptococcus iniae. In the first trial, the doses of the pathogens for challenge test were determined based on LD50.
View Article and Find Full Text PDFThis study evaluates changes in the pharmacokinetic behavior of a single oral dose of florfenicol in rainbow trouts experimentally infected with Lactococcus garvieae or Streptococcus iniae. One hundred and fifty fish were randomly divided into three equal groups: 1-healthy fish, 2-fish inoculated with S. iniae (2.
View Article and Find Full Text PDFThe purpose of the present study was to isolate and identify the pathogenic agents in (Pallas, 1771) and (Linnaeus, 1758) reared in the south of Fars province, Iran which have shown infectious disease signs. Samples from spleen and kidney of 32 fishes showing septicemia symptoms such as decreasing of appetite, unbalanced swimming, expanded wounds, and petechia on the body surfaces, pectoral fins rot, visceral hemorrhage, bleeding on the spleen, and heart ascites were collected. Then samples were cultured on brain heart infusion agar growth media, stain and biological and biochemical tests on purified bacteria were performed.
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