Comparative metabolic profiling and quantitative analysis of metabolites in different tissues of Ajuga turkestanica by ESI-UHPLC-QqTOF-MS and NMR.

Ajuga turkestanica preparations are used as anti-aging cosmeceuticals and for medicinal purposes. Herein we describe the characterization and quantification of its metabolites in different organs using UHPLC-MS and NMR spectroscopy. A total of 51 compounds belonging to various phytochemical classes (11 flavonoids, 10 ecdysteroids, 9 diterpenes, 6 fatty acids, 5 iridoids, 3 phenylpropanoids, 3 sugars, 2 phenolics, 1 coumarin, 1 triterpene) were annotated and tentatively identified by UHPLC-ESI-QqTOF-MS/MS of methanolic extracts obtained separately from the organs.

Therapeutic potential of boswellic acids: an update patent review (2016-2023).

Introduction: Boswellic acids (BAs) are a group of pentacyclic triterpenoids of the ursane and oleanane type. They have shown very interesting biological properties that have led to the development of a number of synthesis protocols. Both natural BAs and their synthetic derivatives may be useful in the treatment of a variety of cancers, viral infections and inflammatory diseases.

Antimicrobial Action Mechanisms of Natural Compounds Isolated from Endophytic Microorganisms.

Infectious diseases are a significant challenge to global healthcare, especially in the face of increasing antibiotic resistance. This urgent issue requires the continuous exploration and development of new antimicrobial drugs. In this regard, the secondary metabolites derived from endophytic microorganisms stand out as promising sources for finding antimicrobials.

Silviridoside: A New Triterpene Glycoside from with Promising Antioxidant and Enzyme Inhibitory Potential.

A new triterpene glycoside, silviridoside, was isolated from the aerial parts of (Caryophyllaceae) using different chromatographic techniques. The structure of silviridoside was comprehensively elucidated as 3-β-D-galacturonopyranosyl-quillaic acid 28--β-D-glucopyranosyl-(1→2)-[α-L-rhamnopyranosyl-(1→3)]-β-D-fucopyranosyl ester by one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HR-MS). Silviridoside showed promising antioxidant activity in different antioxidant assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) (2.

GC-MS Chemical Profiling, Biological Investigation of Three Species Growing in Uzbekistan.

is a potentially valuable aromatic herb that has been used since ancient times. The present work studied the chemical profile of three species essential oils (EO): , and , as well as assessing their antioxidant and enzyme inhibitory activities. A total of 144 compounds were detected by GC-MS analysis, representing 91.

Ecdysteroids as Potent Enzyme Inhibitors and Verification of Their Activity Using In Vitro and In Silico Docking Studies.

Ecdysteroids represent arthropods' steroidal hormones, and they exist in about 5-6% of plant species. In this study, the enzyme inhibitory activity of 20 ecdysteroids was assessed for the first time via determining their inhibition versus acetylcholinesterase, butyrylcholinesterase, tyrosinase, as well as α-amylase enzymes. Furthermore, 20-Hydroxyecdysone-2,3,22-tri--acetate () showed the highest inhibition of acetylcholinesterase and butyrylcholinesterase with values of 5.

Fruit Peels: Food Waste as a Valuable Source of Bioactive Natural Products for Drug Discovery.

Fruits along with vegetables are crucial for a balanced diet. These not only have delicious flavors but are also reported to decrease the risk of contracting various chronic diseases. Fruit by-products are produced in huge quantity during industrial processing and constitute a serious issue because they may pose a harmful risk to the environment.

Chemometric Analysis Based on GC-MS Chemical Profiles of Three Species from Uzbekistan and Their Biological Activity.

The chemical composition of the essential oils (EOs) of , and growing in Uzbekistan were determined, and their antioxidant and enzyme inhibitory activity were assessed. A gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of 143 metabolites accounting for 70.34, 76.

Discrimination of the Essential Oils Obtained from Four Apiaceae Species Using Multivariate Analysis Based on the Chemical Compositions and Their Biological Activity.

The chemical composition of the essential oils obtained from the aerial parts of four Apiaceae species, namely (EA), (EP), (FC), and (HI), were determined using gas chromatography. Altogether, 100 volatile metabolites representing 78.97, 81.

Meroterpenoids: A Comprehensive Update Insight on Structural Diversity and Biology.

Meroterpenoids are secondary metabolites formed due to mixed biosynthetic pathways which are produced in part from a terpenoid co-substrate. These mixed biosynthetically hybrid compounds are widely produced by bacteria, algae, plants, and animals. Notably amazing chemical diversity is generated among meroterpenoids via a combination of terpenoid scaffolds with polyketides, alkaloids, phenols, and amino acids.

Sugar Containing Compounds and Biological Activities of .

Phytochemical investigation of the methanolic extract obtained from the aerial parts of (Lamiaceae) afforded the new compound 1-methoxy-3---glucopyranosyl-α-l-oliose () together with five known glycosides, namely sitosterol-3---glucoside (), stigmasterol-3---glucoside (), pinitol (), 6-hydroxyl-7--loganin (), and chlorotuberoside (). The structures of these compounds were elucidated by extensive spectroscopic analyses, especially HR-MS, 1D and 2D NMR spectroscopy. The in vitro cytotoxic activity of the methanolic extract and the isolated compounds was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and crystal violet (CV) staining assays.

The Genus (Lamiaceae): A Review of Its Diversity, Ethnobotany, Phytochemistry, and Pharmacology.

The genus (Lamiaceae) is native to Central, South-Central, and Eastern Asia. It comprises 44 species, which have been commonly used as herbal medicines for the treatments of various ailments for thousands of years, especially in Asian countries. This review aims to summarize the chemical constituents and pharmacological activities of species from the genus to unveil opportunities for future research.

GC-MS Based Identification of the Volatile Components of Six Species from Uzbekistan and Their Biological Activity.

The compositions of volatile components in the aerial parts of six species, namely (), (), (), (), () and (), were investigated using gas chromatograph-mass spectrometry (GC-MS) analysis. Ninety-seven metabolites were identified, accounting for 73.28, 87.

Diterpenes from an Uzbek medicinal plant Perovskia scrophulariifolia: Their structures and anti-neuroinflammatory activity.

Phytochemical investigation on the aerial parts of a Lamiaceous medicinal plant Perovskia scrophulariifolia collected in Uzbekistan resulted in the isolation of two new 20-norabietane diterpenes, along with thirteen known diterpenes including one 20-norabietane, eight abietanes, one 6,7-secoabietane, and three icetexanes. The structures of new 20-norabietane diterpenes, perovsfolins C (1) and D (2), were elucidated by spectroscopic analyses aided with calculations of ECD spectra. Perovsfolin C (1) is the first 20-norabietane diterpene possessing a 1,11-epoxy moiety, while perovsfolin D (2) is a 20-norabitetane diterpene with a 2-hydroxy-1,4-quinone moiety as C-ring.

Chemical Profiling and Discrimination of Essential Oils from Six Species Using GC Analyses Coupled with Chemometrics and Evaluation of Their Antioxidant and Enzyme Inhibitory Potential.

The differences in the composition of essential oils obtained from the aerial parts of six species viz., (), (), (), (), () and () were detected both qualitatively and semi-quantitatively using GC-MS and GC-FID analyses. One hundred and six metabolites were identified that account for 92.

Comparative study on the chemical composition and biological activities of the essential oils of three species collected from Uzbekistan.

species are mainly distributed in Central Asia and widely used in folk medicine as a sedative and haemostatic. The present investigation reports on the extraction by hydrodistillation and the chemical composition of three species ( and ) essential oils from Uzbekistan. The chemical composition of these essential oils was determined by GC-MS.

α-Ecdysone suppresses inflammatory responses via the Nrf2 pathway in lipopolysaccharide-stimulated RAW 264.7 cells.

The search for new biologically active compounds is a topic of current research because of their ubiquitous availability and low toxicity. Plants of the Silene genus contain secondary metabolites known as phytoecdysteroids that reportedly have various biological activities. α-Ecdysone is a phytoecdysteroid with biological activity that has not been thoroughly investigated to date.

A comparative study on chemical composition and antimicrobial activity of essential oils from three species from Uzbekistan.

Essential oils obtained from the aerial parts of and were determined using GC-FID and GC-MS methods. A total of 76 components were identified in the three species representing 97.12, 88.