Multimodal evaluation of ultra-short laser pulses treatment for skin burn injuries.

Thousands of people die every year from burn injuries. The aim of this study is to evaluate the feasibility of high intensity femtosecond lasers as an auxiliary treatment of skin burns. We used an animal model and monitored the healing process using 4 different imaging modalities: histology, Optical Coherence Tomography (OCT), Second Harmonic Generation (SHG), and Fourier Transform Infrared (FTIR) spectroscopy.

D-Scan measurement of ablation threshold incubation effects for ultrashort laser pulses.

We report the validation of the Diagonal Scan (D-Scan) technique to determine the incubation parameter for ultrashort laser pulses ablation. A theory to calculate the laser pulses superposition and a procedure for quantifying incubation effects are described, and the results obtained for BK7 samples in the 100 fs regime are compared to the ones given by the traditional method, showing a good agreement.

Morphological assessment of dentine and cementum following apicectomy with Zekrya burs and Er:YAG laser associated with direct and indirect Nd:YAG laser irradiation.

Objectives: This study aimed to assess the apical surface morphology of maxillary central incisors resected 3.0 mm from the tooth apex using Zekrya burs or Er:YAG laser, with or without subsequent direct Nd:YAG laser irradiation (apical and buccal surfaces) and indirect irradiation (palatal surface).

Study Design: Forty maxillary central incisors were instrumented and obturated.

Prospective ultramorphological characterization of human hair by optical coherence tomography.

Background/purpose: The continuous advancement in cosmetic science has led to an increasing demand for the development of non-invasive, reliable scientific techniques directed toward claim substantiation, which is of utmost relevance, to obtain data regarding the efficacy and safety of cosmetic products.

Methods: In this work, we used the optical coherence tomography (OCT) technique to produce in vitro transversal section-images of human hair. We also compared the OCT signal before and after chemical treatment with an 18% w/w ammonium thioglycolate solution.

Analytical quantification of low-density lipoprotein using europium tetracycline indicator.

Low-density lipoprotein (LDL) is known as 'bad' cholesterol. If too much LDL circulates in the blood it can be retained in the walls of the arteries, causing atherosclerosis. In this paper we showed an alternative method to quantify LDL using the europium tetracycline (EuTc) indicator.

Correlation between autofluorescence intensity and tumor area in mice bearing renal cell carcinoma.

Protoporphyrin IX (PpIX) is a porphyrin derivative that is accumulated in cancerous tissue in consequence of the tumor-specific metabolic alterations. The aim of this study was to evaluate the accumulation of PpIX in mice bearing renal cell carcinoma by spectroscopy analysis. A total of 24 male Balb/c mice, 6 weeks old, were divided into six groups: Normal (without inoculation of tumor cells) and 4, 8, 13, 16, and 20 days after inoculation of tumor cells.

Study of blood porphyrin spectral profile for diagnosis of tumor progression.

Renal cell carcinoma (RCC) accounts for approximately 3% of new cancer incidence and mortality in the United States. Unfortunately many RCC masses remain asymptomatic and nonpalpable until they are advanced. Diagnosis and localization of early carcinoma play an important role in the prevention and curative treatment of RCC.

Enhancement of europium luminescence in tetracycline-europium complexes in the presence of urea hydrogen peroxide.

An increase in the europium emission band was observed, for the first time, with addition of urea hydrogen peroxide to the tetracycline-europium (Tc-Eu)solution. We have observed that the wavelength, the band width and the area of 5D0-->7F2 europium transition change with the urea hydrogen peroxide concentration. We claim that the tetracycline-europium complexes can be used as probes of urea hydrogen peroxide concentration.