Pulmonary vasculature development in congenital diaphragmatic hernia: a novel automated quantitative imaging analysis.

Purpose: Impaired fetal lung vasculature determines the degree of pulmonary hypertension in the congenital diaphragmatic hernia (CDH). This study aims to demonstrate the morphometric measurements that differ in pulmonary vessels of fetuses with CDH.

Methods: Nitrofen-induced CDH Sprague-Dawley rat fetuses were scanned with microcomputed tomography.

The Survivorship Bias in Congenital Diaphragmatic Hernia.

Current literature for congenital diaphragmatic hernia (CDH) focuses on the comparison of the overall mortality in CDH patients. Only a few studies concentrate on analyzing the unstable patients who could not achieve surgical repair, as well as those who could but did not survive after. Hence, this study aimed to analyze the effects of various parameters on the timing of death.

Reversible Fetal Tracheal Occlusion in Mice: A Novel Minimal Invasive Technique.

Background: There is a certain need for reversible, cheap, and reproducible animal models for understanding the impact of tracheal occlusion (TO) in the congenital diaphragmatic hernia and pathophysiology. We aimed to present an easy, reversible, and minimally invasive murine TO model with optimized time points for introduction and removal of TO.

Methods: Time-mated C57BL/6 mice underwent laparotomy at embryonic day 16.