Publications by authors named "Nikolett Zsibrita"
Targeted DNA methylation is a technique that aims to methylate cytosines in selected genomic loci. In the most widely used approach a CG-specific DNA methyltransferase (MTase) is fused to a sequence specific DNA binding protein, which binds in the vicinity of the targeted CG site(s). Although the technique has high potential for studying the role of DNA methylation in higher eukaryotes, its usefulness is hampered by insufficient methylation specificity.
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- Researchers developed the I-Block assay, a straightforward method for detecting specific protein-DNA binding in E. coli.
- The assay uses two plasmids and an E. coli strain lacking the lacI gene, where one plasmid has a modified lacI promoter allowing for testing of protein binding.
- It was successfully tested with various proteins, demonstrating effectiveness in different culture setups and showing increased β-galactosidase activity when proteins bind to the promoter.
The highly similar prokaryotic DNA (cytosine-5) methyltransferases (C5-MTases) M.MpeI and M.SssI share the specificity of eukaryotic C5-MTases (5'-CG), and can be useful research tools in the study of eukaryotic DNA methylation and epigenetic regulation.
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