Efficient Cryopreservation of by In Vitro-Grown Axillary Buds and Genetic Stability of Recovered Plants.

Axillary buds of in vitro microshoots were successfully frozen at -196 °C by the one-step freezing method using the protective vitrification solution 2 (PVS2). Microshoots were taken from 11 transgenic lines and three wild type lines. Influence of different explant pretreatments were analyzed from the point of their influence towards recovery after cryopreservation.