Publications by authors named "Nikolay Karnaukhov"
Purpose: Determining the primary origin of non-organ-confined neuroendocrine tumors (NETs) for accurate diagnosis and management. Neuroendocrine tumors are rare neoplasms with diverse clinical behaviors. Determining their primary origin remains challenging in cases of non-organ-confined NETs.
View Article and Find Full Text PDFPancreatic neuroendocrine tumors (NETs) are rare well-differentiated neoplasms with limited therapeutic options and unknown cells of origin. The current classification of pancreatic neuroendocrine tumors is based on proliferative grading, and guides therapeutic strategies, however, tumors within grades exhibit profound heterogeneity in clinical manifestation and outcome. Manifold studies have highlighted intra-patient differences in tumors at the genetic and transcriptomic levels.
View Article and Find Full Text PDFA biosynthetic gene cluster for the bioactive fungal sesterterpenoids variecolin () and variecolactone () was identified in ATCC 16872. Heterologous production of and was achieved in by expressing the sesterterpene synthase VrcA and the cytochrome P450 VrcB. Intriguingly, the replacement of VrcB with homologous P450s from other fungal terpenoid pathways yielded three new variecolin analogues (-).
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- Pancreatic fibrosis (PF) is key in chronic pancreatitis and this study examined its relationship with various factors in pancreatic specimens from 74 patients.
- Significant differences were found in imaging parameters (like unenhanced pancreas density and contrast enhancement ratio) based on fibrosis grades and inflammation indicators in the tissue.
- Blood levels of fibronectin and hyaluronic acid showed correlations with different aspects of fibrosis, suggesting that these and imaging parameters could aid in non-invasive diagnosis of PF.
Background: Gastrointestinal stromal tumors (GISTs) are the most frequent mesenchymal tumors of gastrointestinal tract. The most common sites of metastases are the liver and the peritoneum, whereas breast metastases from GIST are extremely rare. We present a second case of GIST breast metastasis.
View Article and Find Full Text PDFBackground: The RNA-binding protein Musashi-2 (MSI2) controls the translation of proteins that support stem cell identity and lineage determination and is associated with progression in some cancers. We assessed MSI2 as potential clinical biomarker in colorectal cancer (CRC) and tubulovillous adenoma (TA) of colon mucosa.
Methods: We assessed 125 patients, of whom 20 had polyps of the colon (TAs), and 105 had CRC.
Article Synopsis
- Current assessment methods for penile cavernous fibrosis in animal models are limited, prompting the study to evaluate micro-computed tomography (micro-CT) as a more effective alternative.
- The study used 25 male rabbits with induced testosterone deficiency, assessing changes in penile samples over a period of 84 days through various analysis methods, including 3D modeling and tissue morphometry.
- Results indicated that micro-CT provided detailed visualization and changes in tissue density, with significant alterations in smooth muscle/connective tissue and collagen ratios observed at different time points, suggesting a relationship between gray value changes and tissue reorganization in fibrosis.
Article Synopsis
- * Researchers found that when MSI2 is removed, it lowers the levels of another protein called EGFR, which is important for cancer cell growth.
- * Depleting MSI2 makes NSCLC cells, especially those with changes in EGFR, less able to grow and makes treatments that target EGFR more effective.
Micro-CT visualization allows reconstruction of eye structures with the resolution of light microscopy and estimation of tissue densities. Moreover, this method excludes damaging procedures and allows further histological staining due to the similar steps in the beginning. We have shown the feasibility of the lab-based micro-CT machine usage for visualization of clinically important compartments of human eye such as trabecular outflow pathway, retina, iris and ciliary body after pre-treatment with iodine in ethanol.
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