Publications by authors named "Nikolay A Shmakov"

Article Synopsis
  • Plant surface properties are vital for coping with environmental stresses, and the cuticle's outer layer is made up of diverse waxes influenced by specific gene regulators.
  • In this study, researchers used a technique called RNA-guided Cas9 endonuclease to create mutations in four barley genes linked to cuticle properties, identifying one gene responsible for a glossy sheath phenotype due to a deficiency in β-diketones.
  • The results indicated that the WIN1 transcription factor plays a crucial role in regulating the biosynthesis of β-diketones in barley by activating certain genes during specific developmental stages and in particular plant organs.
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  • Potatoes are a globally significant crop, and studying their genomic sequences helps understand their diversity and evolutionary variations.
  • Researchers sequenced the genomes of 15 Russian potato cultivars and compared them to 12 South American varieties, finding differences in genetic diversity and copy number variations (CNVs).
  • Notable discoveries include genes linked to immune responses, tuber development, and a potential new gene related to circadian rhythm regulation in Russian potatoes.
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The size, shape, and color of wheat seeds are important traits that are associated with yield and flour quality (size, shape), nutritional value, and pre-harvest sprouting (coat color). These traits are under multigenic control, and to dissect their molecular and genetic basis, quantitative trait loci (QTL) analysis is used. We evaluated 114 recombinant inbred lines (RILs) in a bi-parental RIL mapping population (the International Triticeae Mapping Initiative, ITMI/MP) grown in 2014 season.

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Barley ( L.) grain pigmentation is caused by two types of phenolic compounds: anthocyanins (which are flavonoids) give a blue or purple color, and melanins (which are products of enzymatic oxidation and polymerization of phenolic compounds) give a black or brown color. Genes and determine the synthesis of purple anthocyanins in the grain pericarp, whereas melanins are formed under the control of the gene in hulls and pericarp tissues.

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  • Globodera rostochiensis is a significant potato pathogen that interacts with host plant roots, with commercial varieties relying on specific resistance genes (R genes) from wild relatives and Andean native potatoes.
  • Research on South American Solanum phureja accessions shows that resistant genotypes trigger an active stress response, including gene activation, tissue regeneration, and hypersensitive responses when attacked by G. rostochiensis.
  • The study highlights the importance of analyzing gene expression changes in S. phureja to understand resistance mechanisms and suggests that de novo transcriptome assembly can identify new resistance traits.
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  • * A study comparing the root transcriptomes of potato genotypes revealed differentially expressed genes related to resistance against a specific nematode, but limited annotations made it difficult to compile a clear list of candidate R genes.
  • * Integrating transcriptomic analysis with data on certain potato genes improved candidate gene identification, suggesting that this combined approach could expedite the discovery of potato R genes for further research and breeding efforts.
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Background: Some plant species have 'melanin-like' black seed pigmentation. However, the chemical and genetic nature of this 'melanin-like' black pigment have not yet been fully explored due to its complex structure and ability to withstand almost all solvents. Nevertheless, identification of genetic networks participating in trait formation is key to understanding metabolic processes involved in the expression of 'melanin-like' black seed pigmentation.

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