Publications by authors named "Nikolaos Mougios"
Article Synopsis
- Fluorescence microscopy has advanced to subnanometer resolution but struggles to visualize single proteins or small complexes; researchers have developed a method called ONE microscopy to address this.
- ONE microscopy expands specimens, tags them with fluorophores, and captures videos to analyze fluorescence fluctuations, allowing for the visualization of individual proteins' shapes at around 1-nm resolution.
- This technique can observe protein conformational changes and has potential applications in clinical settings, such as analyzing protein aggregates in cerebrospinal fluid from Parkinson's patients, bridging high-resolution biology and light microscopy for new discoveries.
Article Synopsis
- Fluorescence microscopy has been a key technique in biological sciences, but traditional methods are often limited to a few targets using primary and fluorescently conjugated secondary antibodies.
- New super-resolution techniques like Exchange-PAINT and SUM-PAINT allow for higher multiplexing but require specialized equipment and expertise.
- The newly developed NanoPlex method improves multiplexing accessibility by using engineered secondary nanobodies to selectively remove fluorescence signals, allowing for the analysis of up to 21 targets in 3D confocal imaging and 5-8 targets in advanced super-resolution techniques.
The structure and dynamics of F-actin networks in the cortical area of B cells control the signal efficiency of B-cell antigen receptors (BCRs). Although antigen-induced signaling has been studied extensively, the role of cortical F-actin in antigen-independent tonic BCR signaling is less well understood. Because these signals are essential for the survival of B cells and are consequently exploited by several B-cell lymphomas, we assessed how the cortical F-actin structure influences tonic BCR signal transduction.
View Article and Find Full Text PDFImaging of living synapses has relied for over two decades on the overexpression of synaptic proteins fused to fluorescent reporters. This strategy alters the stoichiometry of synaptic components and ultimately affects synapse physiology. To overcome these limitations, here a nanobody is presented that binds the calcium sensor synaptotagmin-1 (NbSyt1).
View Article and Find Full Text PDFExpansion microscopy (ExM) improves imaging quality by physically enlarging the biological specimens. In principle, combining a large expansion factor with optical super-resolution should provide extremely high imaging precision. However, large expansion factors imply that the expanded specimens are dim and are therefore poorly suited for optical super-resolution.
View Article and Find Full Text PDFThe function of the postsynaptic compartment is based on the presence and activity of postsynaptic receptors, whose dynamics are controlled by numerous scaffolding, signaling and trafficking proteins. Although the receptors and the scaffolding proteins have received substantial attention, the trafficking proteins have not been investigated extensively. Their mobility rates are unknown, and it is unclear how the postsynaptic environment affects their dynamics.
View Article and Find Full Text PDFDNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. Here, we combine fluorescence lifetime imaging microscopy (FLIM) with DNA-PAINT and use the lifetime information as a multiplexing parameter for targets identification.
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