Publications by authors named "Nikolaos G Tairis"
Article Synopsis
- The human major histocompatibility complex (MHC) is a crucial part of the immune system, located on Chromosome 6, and is involved in various health traits and diseases, but it's complex to study.
- A new method using long-read sequencing technologies allows for precise targeted sequencing and haplotypic assembly of the MHC region in samples with two different alleles.
- The approach has been tested successfully, showing high coverage and accuracy, making it a cost-effective alternative to whole-genome sequencing that could advance research in immunology and genetics.
Summary: A number of methods have been devised to address the need for targeted genomic resequencing. One of these methods, region-specific extraction (RSE) is characterized by the capture of long DNA fragments (15-20 kb) by magnetic beads, after enzymatic extension of oligonucleotides hybridized to selected genomic regions. Facilitating the selection of the most appropriate capture oligos for targeting a region of interest, satisfying the properties of temperature (Tm) and entropy (ΔG), while minimizing the formation of primer-dimers in a pooled experiment, is therefore necessary.
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