Publications by authors named "Nikolai I Strizhov"
Biochemistry (Mosc)
September 2023
Article Synopsis
- Cytochrome CYP102A1 (P450 BM3) from Priestia megaterium is a promising candidate for directed evolution due to its unique functions and ability to hydroxylate androstanes into bioactive products.
- The study focused on expressing a mutant form of this enzyme, CYP102A1-LG23, in Mycolicibacterium smegmatis BD, achieving successful conversion of androst-4-ene-3,17-dione into 7β-OH-AD.
- Co-expressing the mutant enzyme with glucose dehydrogenase (GDH) led to a significant increase in hydroxylation yield, demonstrating the potential for engineered bacteria to produce valuable steroid compounds.
