Impact of cultivation factors in vitro on the growth and the biosynthesis of steviol glycosides in cell cultures.

The growth and the biosynthesis of steviol glycosides (SGs) in cell cultures subjected to the impact of different cultivation factors were investigated under in vitro conditions. The changes in composition and concentration of constituted components of the nutrient medium was found to exert a significant influence on the of cell culture growth and the biosynthesis of SGs. It was found that combination of growth regulators in the nutrient medium is more important factor for proliferation of isolated cells of while the level of SGs accumulation depends to greater extent on genotypical features of cell strain.

Features of development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides.

Growth and development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides (SGs) were investigated. It was found that, owing to the highly favorable conditions of shoot cultivation created in such an apparatus, the intensity of shoot growth and SG production appeared to be 1.5 - 2.