[The relationship between the conditions of cultivation and sporification of various Bacillus strains and the results of the evaluation of the sporocidal activity of chemical desinfectants].

The researchers compared the main microbiological characteristics of some Bacillus antracoides strains after their cultivation in different nutrient media. The study found that the growth and sporification rate, R-S dissociation ability, and sensitivity to disinfectants are strain properties and significantly depend on the composition of the nutrient medium.

[A research into the possibility of the application of new peroxosolvate-based compounds as chemical disinfectants].

A newly synthesized peroxosolvate, ammonium sulphate peroxosolvate, can be used in disinfectology and other areas along with other hard forms of hydrogen. Optimal conditions for ammonium sulphate peroxosolvate synthesis were determined by this study. It was established that (NH4)2SO4 .

[The development of peroxosolvate-based composites and the evaluation of their effectiveness against extremely dangerous infective pathogens].

The article contains the results of the test of PFK-M composition bactericidal activity against a range of extremely dangerous infective pathogens. The preparation proved to be highly effective for the disinfection of surfaces, sanitary and technical equipment, linen, dishes, and medical utensils contaminated by plague, cholera, and anthrax pathogens. The study found that the antimicrobial activity of the preparation increased after heating and lowered when the object to be disinfected was contaminated with protein admixtures.

[A research into the possibility of biological control over technophilic microorganisms using KSAP-U, a military device for biological reconnaissance].

The paper is dedicated to the detection of technophilic microorganisms, able to affect fuels, lubricants, and construction materials. The study demonstrated that indicator paper used in KSAP-U, a biological reconnaissance device, was able to detect technophilic test microorganisms in samples of rubber, turbonit, and the water phase of the "fuel-medium" system even when the degree of material affection was minimal. The authors suggest the practical application of these means for the express indication of technophilic microorganisms in technical objects.

[Impact of endogenous porphyrin-photosensitized exposure on peripheral lymphocytes in patients with pulmonary tuberculosis].

Peripheral lymphocytes (PL) were studied in 63 patients with pulmonary tuberculosis and 28 donors. The study revealed the elevated levels of lipid peroxidation products, altered membrane potential, and diminished PL viability in patients with pulmonary tuberculosis as compared with healthy donors. Analysis of the time dependences of cell viability and production of active tuberculosis products after endogenous porphyrin-photosensitized exposure suggests that the lymphocytes from patients with pulmonary tuberculosis are more exposed to the damaging effect of free radicals than the cells from healthy individuals.

[The sporicidal activity of disinfectants based on hydrogen peroxide].

The authors analyzed the experience of disinfection experts related with the designing of new disinfection chemicals and of their composition variations. The key regularities were defined, which are important for the designing of effective disinfectants with preset properties capable of rapidly and safely inactivating a microbe contamination of different objects in the human habitat. A high antimicrobial activity and good outlooks are demonstrated for disinfectants made on the basis of hydrogen peroxide.

[Chemicals for disinfection and decontamination used and being designed for eliminating the aftermaths of biological contamination].

The efficiency of the key classes of disinfecting and decontaminating chemicals, used to eliminate a biological contamination of the human habitat, was analyzed in the paper. It is underlined that, should any biological agents with unknown properties (biological terror, military threat) be detected, it is advisable to choose the ecologically less hazardous disinfectants based on hydrogen peroxide, aldehydes and chlorine-containing compounds.

[Preparation and use of chemical disinfectants in biological protection and prevention of contagious diseases : ecological aspects].

Ecological requirements to reduce negative effects of chemical disinfectants were one on major determinants the line of works to develop and introduce novel compounds that are environmentally safe hydrogen peroxide derivatives. The paper analyzes the possible routes of entering different fluorine compounds into the environment when disinfecting measures are made at therapeutical-and-preventive institutions, when emergencies occur at microbiological synthesis plants. The maximum concentration of a chemical that has no effect on biochemical processes when it is continuously used at sewage works is chosen as a safe (permissible) level.

[Problems of disinfection and decontamination in the biological protection and prevention of contagious diseases].

The problems of biological protection of the general population and military staff against biological hazard sources, primarily against biological terrorism and military threat underlie works on the design of new disinfectants having a universal spectrum of antimicrobial action and less ecological hazard than currently available ones. Elimination of biological hazard require effective chemical disinfectants that can completely inactivate microbes of unknown etiology as rapidly as possible and that cause the minimum damage to environmental objects. The disinfectant PFK of various modifications is evaluated in terms of the requirements of that ecological and chemical concept aimed at testing chemicals entering the environment.

[The development of a technology for producing a new disinfecting agent with a universal spectrum of antimicrobial action].

The paper summarizes the findings of the physicochemical and potency of potassium fluoride peroxohydrate (PFP-1), a new Russian disinfecting agent having a universal spectrum of antimicrobial effects. It also summarizes the results of long-term practical use for the routine and final disinfection of rooms, sanitary engineering equipment, glassware, medical instruments against both sanitary indicative microorganisms, and actual pathogens of disease in infective and somatic hospitals, tuberculous dispensaries, sanatoria, schools, kindergartens, military hospitals, maternity houses of Russian different cities, towns, and localities. The paper presents comparative data on the mechanism of action of various modifications of PFP on microbial spores, on the stability of agents and their solutions during storage, on the damaging activity on the materials of the processed objects, on toxicological and ecological risks.

[The group identification of the causative agents of infectious diseases in environmental objects].

Drop colorimetric reactions were studied for group identification of microorganisms referring to as viral rickettsial bacterial vegetative and bacterial sporal taxonomic groups. For their determination, the authors proposed a number of well-known reactions for preliminary detection of microorganisms if there was protein or protein components in a sample, as well as a series of reactions characteristic of some groups of microorganisms and their vital activity. The list was proposed to include not only tests for dehydrogenase and peroxidase activities and for inorganic phosphates, but also those for the ability of suspension production, etc, which were specific signs of the presence and vital ability of microorganisms.

[Clinico-immunological studies on the therapeutic efficacy of Immunal in acute bronchitis in children frequently and persistently ill].

Efficacy was studied of the use of the drug preparation "Immunal" (LEK, Slovenia [correction of Slovakia]) in a combined treatment of acute bronchitis in children in poor health. The main group of 39 children received conventional treatment supplemented with a course of the drug "Immunal", 10 drops on a three-times daily schedule over 30 days. The use of "Immunal" in a complex therapy of acute bronchitis in children made for a sooner disappearance of clinical manifestations of the illness, decline in ESR, rise in IgG.

[Fibrinolytic activity of the lacrimal fluid in disordered choroid circulation in patients with arterial hypertension].

Fluorescent angiographic examination of the choroid and retinal circulation in 43 patients with arterial hypertension of different origin without signs of hypertensive retinopathy with low and normal values of the fibrinolytic activity of the lacrimal fluid (FALF) revealed a correlation between disorders of the choroid circulation (ischemic zones, delayed time of contrast staining of the choroid) and reduction of FALF. The results indicate that a reduction of FALF may be regarded as a prerequisite for the development of circulatory disorders in the choroid.

[The effect of cytoskeleton modification on the process of trypsin-induced cell aggregation].

Studies were performed on the influence of cytoskeleton modification (by colchicine, vinblastine, and cytochalasin B, and by neoplastic transformation) on cell aggregation induced by small trypsin concentrations (the final concentrations equal to 1-100 mg/ml) added to a suspension of chick embryo fibroblasts dispersed from a monolayer with EDTA. It was shown that alteration of microfilaments resulted in the loss of the ability of plasma membranes to structural rearrangements. It is suggested that aggregation of these cells is realized due to a gradual exposure of adhesive sites along with proteolytic splitting of protein components of plasma membranes.

[Local fibrinolytic activity of the lacrimal fluid in patients with arterial hypertension].

Lacrimal fluid local fibrinolytic activity was measured in 101 patients with different variants of arterial hypertension. The studied parameter was found reduced in patients developing hypertensive retinopathy on the respective eye. A correlation was revealed between progress of hypertensive changes in the fundus oculi and reduction of lacrimal fluid local fibrinolytic activity.

[The effect of antiaggregants on the course of diabetic microangiopathy].

As shown by conjunctival biomicroscopy, 6-month administration of ticlid and gliclazide induced a statistically significant increase in the frequency of less severe microcirculatory disturbances compared to conventional hypoglycemic therapy. The drugs also restricted plasmatic impregnation and cell proliferation in skin biopsy microvascular wall. Following 2-year antiaggregation treatment (gliclazide, trental, dipiridomol) 48.

[Interaction of bromophenol blue with serum albumin: criteria for using dyes for assessing the state and quantity of protein].

The optical properties of the complexes of the pH-dependent dye bromophenol blue (BPB) with human serum albumin were investigated by the spectrophotometric method. The solvatochromic longwave displacement of bound BPB-2 absorption and BPB-1/BPB-2 redistribution were shown to form the optical signal of complexes. Because of the distortion of the bound BPB-2 signal its quantity was determined as delta A630 = A630 - A660 and the use of lambda max as structural parameter was limited to low pH less than or equal to 3.

[Structuro-functional characteristics of aldolase from rabbit muscles in diabetes].

The structural peculiarities of rabbit muscle aldolase accompanying enhancement of the aldolase activity in diabetes are described from the data of tryptophan phosphorescence at the room temperature and fluorescence polarization. It is shown that the pathology-concomitant conformational changes occur in both the hydrophobic part and NAD-binding site of the enzyme. The character of the structural changes in the hydrophobic part of the protein in diabetes and an increase in the enzymic activity are similar to that observed in normal aldolase after its interaction with NADH and are believed to be associated with the enhancement of the rigidity in the Trp-147 environment.

[Severe and malignant arterial hypertension: the optimal treatment].

54 patients were treated with a combination of capoten and diuretics for 40 to 250 days. Some patients had been subjected to the "nitroprusside test" prior to medication. The relation between the clinical state, the perilimbal and eye fundus microcirculation and degree of BP decrease were studied using fluorescent angiography.

[Use of the immunofluorescence reaction for evaluating the immunological transformation in those inoculated with a chemical typhus vaccine].

The results of the serological examination of persons immunized with chemical typhus vaccine (CTV) are presented. The examination was carried out by means of the complement fixation test (CFT), the passive hemagglutination test (PHAT), the toxin neutralization test (TNT) and the immunofluorescence test (IFT). The acetone-fixed live culture of Rickettsia prowazekii, strain Breinl, served as antigen in IFT.