Publications by authors named "Nikita A Demidov"
Rational Design of Far-Red Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators: from Elucidation of the Fluorescence Mechanism in Archers to Novel Red-Shifted Variants.
Dmitrii M Nikolaev Vladimir N Mironov Ekaterina M Metelkina Andrey A Shtyrov Andrey S Mereshchenko Nikita A Demidov
ACS Phys Chem Au
July 2024
Article Synopsis
- Genetically encoded voltage indicators (GEVIs), especially those based on archaerhodopsin-3, are useful for visualizing changes in cell membrane potential.
- To improve these tools, researchers aimed to create new GEVIs with better fluorescence and absorption properties.
- The study successfully enhanced the fluorescence of archaerhodopsin-3 by stabilizing specific protein conformations through targeted mutations, leading to the design of new variants with a broader absorption range and significantly brighter emissions.