[Evolution of serologically confirmed cases of mumps in the Madrid community, Spain, during a period of 7 years (2000--2006)].

Background And Objective: The aim of this study was to describe the evolution of serologically confirmed cases of mumps in the Madrid Community from 2000 to 2006.

Patients And Method: Cases with data about age, sex and with samples obtained < or = 30 days after the onset of symptoms were included. IgM determination was performed by indirect ELISA (Enzygnost Mumps; Dade Behring).

[Assessment of two methods of antigenic detection by ELISA for the diagnosis of norovirus outbreaks].

Introduction: The aim of this study was to assess two ELISA techniques for the detection of outbreaks of norovirus.

Method: One-hundred and sixty-five fecal samples from patients affected in 30 outbreaks were studied.

Results: On the basis of a specific consensus criterion between techniques for confirming outbreaks, the sensitivity and specificity was respectively 80% and 90% for RIDASCREEN, and 80% and 100% for IDEIA.

Sensitivity and specificity of immunoglobulin G titer for the diagnosis of mumps virus in infected patients depending on vaccination status.

The aim of the study was to evaluate the usefulness of serological detection of mumps IgM and titration of IgG in patients with acute parotitis according to their vaccination status. The detection of mumps virus RNA in saliva by RT-PCR was used as reference. 116 patients (109 of them previously vaccinated) with mumps RT-PCR-negative results and 21 (19 vaccinated) with mumps RT-PCR-positive results were studied.

[Diagnostic performance of specific IgM detection and genomic amplification in rubella].

Nowadays, most exanthematic diseases for which a vaccine is available affect young adults. A large percentage of these cases prove to be rubella. The aim of this study is to assess the performance of specific IgM and RT-PCR for the diagnosis of rubella infection.