Regional localization of the gene encoding pregnancy specific beta-1-glycoprotein 1 (PSBG1) to human chromosome 19q13.1.

Pregnancy specific beta-1-glycoprotein (PSBG), a major product of the human placenta, is encoded by multiple genes. Southern blot hybridization of human x rodent somatic cell hybrid DNAs with a cDNA specific for one member of the PSBG gene family allowed us to map this gene to human chromosome 19. Further analysis using hybrids with subchromosomal segments of 19q revealed that the gene maps to the distal segment of region 19q13.

Regional localization and characterization of a DNA segment on the long arm of chromosome 21.

A human genomic DNA fragment, pAM37 (HGM8; D21S22), was mapped to chromosome 21q2.1-q2.21 by in situ hybridization.

An estimate of unique DNA sequence heterozygosity in the human genome.

Fifteen different restriction fragment length polymorphisms (RFLPs) were detected in the human genome using 19 cloned DNA segments, derived from flow-sorted metaphase chromosomes or total genomic DNA, as hybridization probes. Since these clones were selected at random with respect to their coding potential, their analysis permitted an unbiased estimate of single-copy DNA sequence heterozygosity in the human genome. Since our estimate (h = 0.

Non-breeding-test methods for dominant skeletal mutations shown by ethylnitrosourea to be easily applicable to offspring examined in specific-locus experiments.

Skeletons of (C3H X 101)F1 mice have been examined in earlier studies of the induction of dominant skeletal mutations. The present experiment was done to determine whether the same criteria used to identify mutations in (C3H X 101)F1 mice could be applied to the offspring collected in specific-locus experiments. Offspring were obtained from an experiment of Hitotsumachi et al.