Effect of Eugenol on Detrusor Muscle: Potential for Overactive Bladder Treatment.

Purpose: This investigation was conducted to elucidate the effects of eugenol on bladder contractility through experimental and in silico approaches.

Methods: To assess the impact of eugenol on muscular contractility, longitudinal strips of bladder tissue, measuring 2 mm by 6 mm, were mounted in perfusion chambers connected to an isometric force transducer. Furthermore, molecular docking studies were conducted to explore the potential of eugenol to target the M3 muscarinic acetylcholine receptor (M3R) and voltage-operated calcium channels (VOCCs) in muscle cells, utilizing in silico techniques.