Chemical Pesticides and Human Health: The Urgent Need for a New Concept in Agriculture.

The industrialization of the agricultural sector has increased the chemical burden on natural ecosystems. Pesticides are agrochemicals used in agricultural lands, public health programs, and urban green areas in order to protect plants and humans from various diseases. However, due to their known ability to cause a large number of negative health and environmental effects, their side effects can be an important environmental health risk factor.

Cosmetics as endocrine disruptors: are they a health risk?

Exposure to chemicals from different sources in everyday life is widespread; one such source is the wide range of products listed under the title "cosmetics", including the different types of popular and widely-advertised sunscreens. Women are encouraged through advertising to buy into the myth of everlasting youth, and one of the most alarming consequences is in utero exposure to chemicals. The main route of exposure is the skin, but the main endpoint of exposure is endocrine disruption.

Sun lotion chemicals as endocrine disruptors.

Ultraviolet solar radiation is a well-known environmental health risk factor and the use of sun lotions is encouraged to achieve protection mainly from skin cancer. Sun lotions are cosmetic commercial products that combine active and inactive ingredients and many of these are associated with health problems, including allergic reactions and endocrine disorders. This review focuses on their ability to cause endocrine and reproductive impairments, with emphasis laid on the active ingredients (common and less common UV filters).

Fibroblast activation protein and its prognostic significance in correlation with vascular endothelial growth factor in pancreatic adenocarcinoma.

Fibroblast activation protein (FAP), a selective protein for tumor stromal fibroblasts, is expressed in >90% of human epithelial carcinomas. A characteristic feature of pancreatic cancer is an extensive fibrotic or desmoplastic reaction surrounding the primary tumor. The present study aimed to evaluate the expression levels of FAP and vascular endothelial growth factor (VEGF) and determine their correlation in pancreatic adenocarcinoma.

Prognostic value of COX-2, P53, and EZH-2 evaluated by quantitative image analysis in premalignant and malignant breast lesions.

Background: Cytological differential diagnosis of atypical hyperplasia and well differentiated breast carcinoma may be challenging, because sometimes there is an overlap between the cytomorphological features of these lesions. The aim of the study was to investigate COX-2, EZH-2, p53 expression in carcinomas and the gray zone of breast cytology categories of atypical hyperplastic lesions with regard to biological behavior of the tumor.

Methods: FNA speciments from 100 patients with breast hyperplastic lesions and cancer were investigated by immunocytochemistry and a quantitative analysis for COX-2, p53, and EZH-2.

Correlation between depression, anxiety, and polymorphonuclear cells' resilience in ulcerative colitis: the mediating role of heat shock protein 70.

Background: To investigate whether anxiety and depression levels are associated with Heat Shock Protein 70 (HSP70) induction in the colon of patients with ulcerative colitis (UC).

Methods: The design was cross-sectional. Clinical activity was assessed by the Rachmilewitz Index (CAI).

Indoor air quality in a bar/restaurant before and after the smoking ban in Athens, Greece.

In this study we compared indoor air pollutant concentrations in a bar/restaurant in Greece before and after the enactment of a smoking ban legislation of 2008. This was done to investigate whether the separation of the venue into smoking and non-smoking areas will have an impact on workers and customers from secondhand smoke (SHS) exposure (null hypothesis). The study was completed within an 8-month period beginning in March 2010 and ending on November 2010.

"Immunocytochemical expression of P53, PTEN, FAS (CD95), P16INK4A and HPV L1 major capsid proteins in ThinPrep cervical samples with squamous intraepithelial lesions".

The aim of this study was to further investigate the immunocytochemical expression of p53, PTEN, Fas, p16, and HPV L1 capsid proteins in cervical smears with low and high grade squamous intraepithelial lesions (LSIL and HSIL, respectively). A total of 92 ThinPrep cervical samples, comprising 11 cases of HSIL, 61 cases of LSIL, and 20 negative cases were studied by immunocytochemical methods. The results obtained in LSIL cases were correlated with the available follow up data.

Children exposure to trace levels of heavy metals at the north zone of Kifissos River.

This study evaluates the exposure level of primary school children at three different towns in northern Attica, near the banks of the Kifissos River, defining referential values of toxic heavy metals. Concentrations of five toxic metals (arsenic, cadmium, mercury, lead and nickel) were analyzed in water samples from the Kifissos River as well as in the scalp hair of children aged 11 to 12 years old living in the study area. Chronic low-level toxicity of lead and cadmium came into view in recent years as a problem of our civilization.

Additional slides from residual ThinPrep Pap tests: Of potential diagnostic benefit in equivocal cases?

The aim of this study was to further evaluate the diagnostic significance of additional slides prepared from residual ThinPrep (TP) Pap Tests. Up to 10 repeat slides were prepared from 105 residual TP cervical samples. All additional slides were evaluated for the presence of diagnostic elements which were not found on the primary TP slide.

Repeat processing of residual ThinPrep Pap tests: sampling of the vial may not be invariably homogeneous.

Objective: To re-evaluate the reproducibility of additional slides prepared from residual cervical ThinPrep (TP) samples.

Study Design: Sixty paired specimens (conventional smears and direct-to-vial TP) were studied. Up to 10 additional slides were prepared from each TP vial.

Enhancer of zeste homologue 2 expression in breast carcinoma smears in relationship with p53, Ki-67 and other prognostic parameters.

Background: The polycomb group protein enhancer of zeste homologue 2 (EZH 2) has been reported as a marker of aggressive breast cancer. The aim of this study was to investigate the correlation between the expression of EZH 2 with p53 and Ki-67 expression and other clinicopathological parameters in primary breast carcinomas in order to determine the role of the above marker as a prognosticator of tumor aggressiveness and patient outcome.

Patients And Methods: One hundred primary operable breast cancer patients were investigated in order to identify the expression of EZH 2, Ki-67 and p53 in imprint smears immunocytochemically.

Subsite-specific differences of estrogen receptor beta expression in the normal colonic epithelium: implications for carcinogenesis and colorectal cancer epidemiology.

Objective: This study aimed at investigating whether a differential estrogen receptor beta (ER-beta) expression between the colonic subsites could correspond to a modification in proliferation, apoptosis, and adhesion of the normal colonocytes.

Methods: ER-beta, Ki-67, Bcl-2, and E-cadherin expressions were investigated immunohistochemically, in normal epithelium biopsies from the ascending and the descending colon of 53 individuals, who underwent colonoscopy for the investigation of anemia and in whom no local pathology was identified.

Results: ER-beta immunoreactivity has been shown to be stronger at the superficial epithelium than the crypts' base, the difference being important only for the ascending colon.

Differential rates of proliferation and apoptosis in nasal polyps correspond to alterations in DNA spatial distribution and nuclear polarization as observed by confocal microscopy.

The study aimed to investigate the expression of p53, Bcl-2 and Ki-67 in relation to the histologic and nuclear qualitative and spatial characteristics of chronic rhinosinusitis with polyposis (CRP). Imprint smears obtained from surgically removed nasal polyps of 20 patients were studied. The polyps were classified according to their histological characteristics as: hyperplasia (simple and pronounced) and squamous metaplasia.

Contribution of nuclear morphometry by confocal laser scanning microscopy to the diagnosis of malignant bile duct strictures.

Objective: To determine the clinical utility of nuclear morphometry by confocal laser scanning microscopy for the diagnosis of malignant biliary strictures.

Study Design: The study included 51 patients with bile duct strictures who underwent endoscopic retrograde cholangiopancreatography (ERCP). Based on the initial workup, 6 patients were diagnosed with benign strictures, and 12 patients had malignant strictures, while in the remaining 33 cases the diagnoses were inconsistent, due mainly to inadequate samples.

Expression of substance P, vasoactive intestinal peptide and heat shock protein 70 in nasal mucosal smears of patients with allergic rhinitis: investigation using a liquid-based method.

Objective: The aim of this study was to investigate expression of the neuropeptides substance P, vasoactive intestinal peptide and heat shock protein 70 in the nasal mucosa cells of patients with seasonal allergic rhinitis, in order to obtain more information on the pathophysiological and immunological role of these markers in allergic rhinitis.

Material And Methods: Nasal epithelium specimens obtained from 42 patients with allergic rhinitis were studied, using Shandon's Papspin liquid-based cytology method. Smears were immunostained with antibodies against substance P, vasoactive intestinal peptide and heat shock protein 70, and the results were correlated with the clinical features of seasonal allergic rhinitis.

Insulinlike growth factor I receptor and estrogen receptor beta expressions are inversely correlated in colorectal neoplasms and affected by the insulin resistance syndrome.

The present study aimed at evaluating the modulation of insulin-like growth factor I receptor (IGF-IR) and estrogen receptor beta (ER-beta) expression and their correlation during tumorigenesis of sporadic colorectal cancer, with particular interest in the insulin resistance syndrome. In a series of 100 individuals (54 men and 46 women; mean age, 67.3 +/- 9.

Exposure to multiple environmental agents and their effect.

Introduction: All children are exposed to multiple physical, chemical and biological challenges that can result in adverse health effects before and after birth. In this context, the danger of multiple exposures cannot be assessed from a single-chemical approach as used in classical toxicology.

Aim: To open up a 'negotiation space' for the problem of multiple exposure to environmental stressors, defined as any physical, chemical or biological entity that can induce an adverse response.

DNA spatial distribution in unfertilized human oocytes by confocal laser scanning microscope.

The aim of this study is to investigate the DNA spatial distribution in unfertilized human oocytes by confocal laser scanning microscope (CLSM). One hundred and forty unfertilized oocytes after conventional in vitro fertilization (IVF) and one hundred and three after intracytoplasmic sperm injection (ICSI) were studied. The chromatin was stained with chromomycin A3 (CA3) initially and at a second step with propidium iodide, allowing the observation of the deprotaminated sperm heads in the ooplasm.

The impact of endocrine disrupters on the female reproductive system.

Over the last decades, many tonnes of man-made chemicals have been produced and released into the environment. Many of these chemical substances have the ability to modulate the action of hormones and are called endocrine disrupters. Cell receptors that have been pure receptors for thousands of years have (due to industrialization), become susceptible to the action of exogenous chemicals.

An international survey of medical school programmes on nuclear war.

Many medical organizations have acknowledged the responsibility of doctors to educate health professionals and the public about the medical, social and ecological consequences of nuclear war. Medical schools have begun the development of programmes on these topics. A total of 1130 medical schools in thirty-one countries were surveyed about their presentation of nuclear-war-related subject matter.

The histologic pattern of bile gastritis.

Various nonspecific histologic findings have been described in gastric biopsies from patients with bile gastritis. In an attempt to identify any characteristic or specific histologic changes in such cases, we have blindly compared gastric biopsies from 23 symptomatic postgastrectomy patients with macroscopic gastritis with biopsies from 17 patients with macroscopic gastritis associated with peptic ulcer. It was shown that in bile gastritis the prominent histologic features were translucency of the surface epithelium, edema of the upper portion of the lamina propria, decrease of the parietal cells, and presence predominantly of neutral mucus in the cells of the surface epithelium and of the neck of the glands.