[Ige reactivity of a recombinant multi-epitope protein designed from allergens of interest in the tropics - preliminary findings].

Objective: The objective of the present study was to design a multi-epitope protein from and APD allergens and to evaluate its IgE reactivity preliminarily.

Methods: Using computational tools, a molecule containing multiple "T" epitopes of allergens derived from and APD was designed "" This multi-epitope protein (MP1) was expressed using an system and purified by affinity chromatography using Ni-NTA agarose. Anti-MP1 and anti-HDM extract IgE reactivity was evaluated by Dot-Blot and indirect ELISA from sera of HDM-allergic patients and non-allergic individuals from Barranquilla-Colombia.

[Novel tropomyosin consensus B and T epitopes involved in cross-reactivity between 10 different species. An in silico study].

Objective: This study aimed to identify by in silico methods tropomyosin consensus B and T epitopes of shrimp species, house dust mites, insects, and nematodes associated with allergic diseases in tropical countries.

Methods: In silico analysis included tropomyosin from mites (Der p 10, Der f 10, Blo t 10), insects (Aed a 10, Per a 7, Bla g 7), shrimp (Lit v 1, Pen m 1, Pen a 1), and nematode (Asc l 3) all sequences were taken from the UniProt database. Linear IgE epitopes were predicted with AlgPred 2.

[IgE reactivity of sera from patients with autoimmune diseases against a multi-epitope protein constructed from T epitopes of Ascaris lumbricoides allergens].

Objective: To evaluate the IgE reactivity of sera in patients suffering from type 1 diabetes (T1D), lupus nephritis (LN) and juvenile idiopathic arthritis (JIA) against a molecule constructed from T epitopes of allergens.

Methods: We designed and expressed a synthetic multi-epítope protein named MP1 from A. lumbricoides and house dust mites allergens.

Distribution of polymorphism rs693 of ApoB gene in a sample of Colombian Caribbeans.

Introduction: Several studies have reported that the single nucleotide polymorphism rs693 of Apo lipoprotein B gene is associated with high levels of plasma lipids and high body mass index, which are risk factors for cardiovascular diseases. The distribution of this single nucleotide polymorphism and its association with the phenotype depend on the genetic background of each population.

Objective: To evaluate the distribution of single nucleotide polymorphism rs693 and its association with lipid profile and body mass index in a sample of Colombian Caribbeans.