Lung cancer cell line screen links fanconi anemia/BRCA pathway defects to increased relative biological effectiveness of proton radiation.

Purpose: Growing knowledge of genomic heterogeneity in cancer, especially when it results in altered DNA damage responses, requires re-examination of the generic relative biological effectiveness (RBE) of 1.1 of protons.

Methods And Materials: For determination of cellular radiosensitivity, we irradiated 17 lung cancer cell lines at the mid-spread-out Bragg peak of a clinical proton beam (linear energy transfer, 2.

Differential bystander signaling between radioresistant chondrosarcoma cells and fibroblasts after x-ray, proton, iron ion and carbon ion exposures.

Purpose: Chondrosarcoma is well known as a radioresistant tumor, but the mechanisms underlying that resistance are still unclear. The bystander effect is well documented in the field of radiation biology. We investigated the bystander response induced by X-rays, protons, carbon ions, and iron ions in chondrosarcoma cells using a transwell insert co-culture system that precludes physical contact between targeted and bystander cells.

Targeted and non-targeted effects from combinations of low doses of energetic protons and iron ions in human fibroblasts.

Purpose: In space, astronauts are exposed to mixed radiation fields consisting of energetic protons and high atomic number, high energy (HZE) particles at low dose rates. Therefore, it is critical to understand effects of combinations of low doses of different radiation types at the cellular level.

Materials And Methods: AG01522 normal human skin fibroblasts and a transwell insert co-culture system were used.