Unanticipated Large-Scale Deletion in Genome Using CRISPR/Cas9 and Its Impact on Growth and Virulence.

, a filamentous fungus, and causal agent of Fusarium head blight (FHB) in wheat and other cereals, leads to significant economic losses globally. This study aimed to investigate the roles of specific genes in virulence using CRISPR/Cas9-mediated gene deletions. Illumina sequencing was used to characterize the genomic changes due to editing.