Publications by authors named "Nicole L Ambrose"
Split aminoacyl-tRNA synthetases for proximity-induced stop codon suppression.
Proc Natl Acad Sci U S A
February 2023
Article Synopsis
- Synthetic biology tools for gene expression regulation primarily focus on transcription, but few target translation; this research introduces split orthogonal aminoacyl-tRNA synthetases (o-aaRS) to fill that gap.
- Using small molecules like rapamycin, these engineered o-aaRSs can turn stop codon suppression on and off, creating a mechanism for controlling gene translation in both bacterial and human cells.
- The study also shows that these split o-aaRSs act as AND gates and can serve as biosensors to monitor important protein interactions linked to cancer and COVID-19.