Publications by authors named "Nicole J Mathewson"
ACS Cent Sci
October 2021
Article Synopsis
- Direct RNA sequencing was used to investigate the epitranscriptomic modification pseudouridine (Ψ) by employing a protein nanopore sensor and a helicase brake to control RNA passage.
- The study discovered that Ψ and uridine (U) can produce different signals in the nanopore sensor depending on their surrounding sequence context, which can lead to biases in sequencing unknown RNA samples.
- To overcome these biases, the researchers found that Ψ's longer dwell time through the helicase brake improved the accuracy of modification detection, leading to the identification of five conserved Ψ sites in the SARS-CoV-2 genome, two of which were validated through additional assays.
