Publications by authors named "Nicole C Elledge"
is a Gram-negative bacterium causally linked to acroporid serratiosis, a form of white pox disease implicated in the decline of elkhorn corals. We report draft genomes of 38 isolates collected from host and nonhost sources. The availability of these genomes will aid future analyses of acroporid serratiosis.
View Article and Find Full Text PDFis a diverse genus of Gammaproteobacteria autochthonous to marine environments worldwide. and were originally isolated from deep-sea hydrothermal fields in the East Pacific Rise. These species are closely related to members of the Harveyi clade (e.
View Article and Find Full Text PDFMembers of the anaerobic gut fungi (AGF) reside in rumen, hindgut, and feces of ruminant and non-ruminant herbivorous mammals and reptilian herbivores. No protocols for gene insertion, deletion, silencing, or mutation are currently available for the AGF, rendering gene-targeted molecular biological manipulations unfeasible. Here, we developed and optimized an RNA interference (RNAi)-based protocol for targeted gene silencing in the anaerobic gut fungus strain C1A.
View Article and Find Full Text PDFArticle Synopsis
- Anaerobic gut fungi (AGF) are a unique fungal group found in the digestive systems of herbivores, reproducing asexually with a development process that includes flagellated zoospores.
- A new optimized method was developed for efficiently collecting viable AGF spores under anaerobic conditions, yielding high quantities of synchronized spores ready for scientific use.
- This method not only allows for the easy recovery and storage of AGF spores without the need for cryopreservation but also ensures their viability for up to 16 weeks at 39°C, making it advantageous for research purposes.