Comparative Study of Eleven Mechanical Pretreatment Protocols for DNA Extraction from Stool Samples.

Nowadays, many commercial kits allow the polymerase chain reaction (PCR) detection of deoxyribonucleic acid (DNA) in stool samples, the efficiency of which relies on the extraction method used. Mechanical pretreatment of the stools using grinding beads has been reported to greatly improve this extraction step. However, optimization of this key step remains to be carried out.

Multicenter Comparative Study of Six DNA Extraction Protocols Including Mechanical Pretreatment from Stool Samples.

Background: Nowadays, many commercial kits allow the detection of sp. in stool samples after deoxyribonucleic acid (DNA) extraction. Protocols of stool pretreatment have been proposed to optimize oocysts' DNA extraction.