Optimizing monosaccharide production from liquid hot water pretreatment and enzymatic hydrolysis of grass-clover press cake.

In the present study, clover-grass press cake was treated by liquid hot water at temperatures of 180-200 °C for a reaction time of 5-10 min. Evaluation of pretreatments was based on the monosaccharide yield after enzymatic hydrolysis of the pretreated slurry and solid fraction, respectively. Extraction of up to 48% hemicellulose and 4% cellulose was observed during pretreatment.

Genetic potential for exopolysaccharide synthesis in activated sludge bacteria uncovered by genome-resolved metagenomics.

A good floc formation of activated sludge (AS) is crucial for solid-liquid separation and production of clean effluent during wastewater treatment. Floc formation is partly controlled by self-produced extracellular polymeric substances (EPS) such as exopolysaccharides, proteins, and nucleic acids. Little is known about the composition, structure, and function of EPS in AS and which bacteria produce them.

Impact of substrate moisture content on growth and metabolic performance of black soldier fly larvae.

Substrate moisture content is an important but not well-understood variable in production and waste reduction processes that involves black soldier fly (BSF) larvae. The purpose of this paper is to characterise growth and metabolic performance of BSF larvae on substrate moisture contents from 45 to 85%. Larvae developed into prepupae only at 45-75% substrate moisture content.

The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.

Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium. Commercially, PDA is available from multiple retailers offering virtually the same product. These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites.