Use of osteogenic bone matrix in patients with traumatic long bone defects: An open label, single center study.

Background: Osteogenic Bone Matrix (Altis™ OBM) is a tissue-engineered, porcine-derived demineralized bone matrix prepared using a humanization processing technology that confers biocompatibility and improved osteoinductivity. The objective of this study was to determine the safety and efficacy of OBM in patients with traumatic long bone defects in an open-label, non-randomized single-center study.

Methods: Diagnosis and main criteria for inclusion were open long bone fractures graded as Gustilo-Anderson Grade II, IIIA or IIIB.

High yield isolation of BMP-2 from bone and in vivo activity of a combination of BMP-2/TGF-β1.

A high-yield purification procedure for protein fractions derived from porcine bone matrix extracts is described, which has a high abundance of bone morphogenetic protein-2 (BMP-2). Naturally derived pBMP-2, ~5 μg per kilogram of porcine bone matrix, was isolated by using a 300 kDa membrane before chromatographic processing on heparin affinity media. The elution of pBMP-2 and transforming growth factor-β(1) (TGF-β(1)) revealed morphogen peaks that were unresolved on Prosep(®) medium, but resolved on hydroxyapatite medium.

Physicochemical modification of kafirin microparticles and their ability to bind bone morphogenetic protein-2 (BMP-2), for application as a biomaterial.

Vacuolated spherical kafirin microparticles with a mean diameter of 5 μm can be formed from an acidic solution with water addition. Three-dimensional scaffolds for hard tissue repair require large structures with a high degree of interconnected porosity. Cross-linking the formed kafirin microparticles using wet heat or glutaraldehyde treatment resulted in larger structures (approximately 20 μm), which, while similar in size and external morphology, were apparently formed by further assisted assembly by two significantly different mechanisms.