Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum beta-lactamases.

The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla(CTX-M) genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on beta-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum beta-lactamase (ESBL) production using phenotypic methods.

The use of NSAIDs in dentistry: a case study of gastrointestinal complications.

Unlabelled: NSAIDs are effective at reducing mild to moderate pain and are therefore commonly prescribed in dental practice. Dental practitioners should be familiar with their indications, contra-indications and side-effects.We present the case of a 28-year-old male who had been taking two NSAIDs concurrently for dental pain which resulted in an acute upper gastrointestinal haemorrhage.