Publications by authors named "Nicholas Donadio"
We present an enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits. Best-in-class vectors were curated for accessing major striatal neuron populations including medium spiny neurons (MSNs), direct and indirect pathway MSNs, as well as Sst-Chodl, Pvalb-Pthlh, and cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes of virus delivery, and with diverse transgene cargos.
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- Researchers addressed the limited access to lower motor neurons (LMNs) in the mammalian spinal cord by creating single cell multiome datasets from mouse and macaque spinal cords to identify enhancers for different neuronal populations.* -
- They cloned identified enhancers into viral vectors and conducted functional tests in mice to screen for effective candidates, which were then validated in rats and macaques.* -
- This new toolkit for labeling LMNs and upper motor neurons (UMNs) can facilitate future research on cell function across species and contribute to potential therapies for neurodegenerative diseases in humans.*
Article Synopsis
- The mammalian cortex consists of different cell types that have specific properties, which are important for understanding how the cortex functions in both health and disease.
- Researchers utilized data from mouse and human studies to identify marker genes and enhancers for various cortical cell types, creating a comprehensive set of tools for targeting these cells specifically.
- They introduced fifteen new transgenic driver lines, two new reporter lines, and over 800 enhancer AAVs, facilitating a wide range of experimental approaches to study the mammalian cortex and its functions.
Article Synopsis
- Recent studies on human cortex have shown that GABAergic neurons have a complex hierarchical organization with various subclasses and specific types.
- Researchers used advanced techniques to study these neurons in human brain slices, combining viral labeling and single-cell RNA sequencing.
- The findings revealed detailed differences within GABAergic neuron types, including variations between human and mouse neurons and highlighted the need for comprehensive analysis to better understand brain cell properties.
The Ca/calmodulin-dependent protein phosphatase calcineurin orchestrates sexual reproduction, stress responses, and virulence via branched downstream pathways in the opportunistic human fungal pathogen The calcineurin-binding protein Cbp1, the calcineurin temperature suppressor Cts1, the calcineurin-responsive zinc finger transcription factor Crz1, and the calcineurin targets Pbp1, Tif3, and Puf4, all function downstream of calcineurin to orchestrate distinct cellular processes. To elucidate how the calcineurin pathway regulatory network governs unisexual reproduction, stress responses, and virulence, we have analyzed the self-filamentous strain, XL280α, and generated double mutants of these calcineurin downstream genes. We demonstrated that calcineurin governs unisexual reproduction at different sexual developmental stages, in which the initiation of the yeast-hyphal morphological transition is independent of Crz1, whereas the sporulation process is dependent on Crz1.
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