Publications by authors named "Nicholas Castro"

Article Synopsis
  • Serology testing is vital for assessing the effectiveness of COVID-19 vaccines and detecting past infections by measuring antibodies against SARS-CoV-2.
  • This study validated four laboratory-developed serology tests for antibodies (IgG and IgM) against Spike and Nucleocapsid proteins, ensuring they met strict Clinical and Laboratory Standards Institute (CLSI) guidelines for reliability.
  • Results showed good linearity and precision across the tests, demonstrating their validity for identifying levels of antibodies, which is crucial for ongoing COVID-19 research and management.
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Article Synopsis
  • SARS-CoV-2 serology is important for assessing COVID-19 vaccine effectiveness and immune response, but factors like tube type and anticoagulant can affect results.
  • The study found no significant differences in antibody levels based on tube type; however, tubes with anticoagulants sodium citrate and ACD showed lower antibody concentrations, which were mostly adjusted after considering dilution factors.
  • There were noticeable differences in IgG avidity between plasma and serum, suggesting that plasma treated with anticoagulant cannot be considered interchangeable with serum for certain assays, warranting further exploration.
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Article Synopsis
  • SARS-CoV-2 vaccination leads to changes in antibody levels and characteristics, which can influence protection against infection.
  • After the initial vaccine series, IgG levels decrease over time while antibody avidity (binding strength) increases; however, this trend is reversed after a third booster dose, which boosts both IgG levels and avidity.
  • Comparison between the two mRNA vaccines shows that one (BNT162b2) results in higher antibody avidity than the other (mRNA-1273) six months after the third dose.
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This study aimed to determine the prevalence of osteoarthritis (OA) and associated clinical signs in young dogs. Owners of dogs aged 8 months-4 years from a single practice, were contacted in random order, to participate in a general health screen. Clinical and orthopedic examinations were performed.

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Background: Cardiometabolic disease (CMD) risk often begins early in life. Healthy lifestyle behaviors can mitigate risk, but the optimal combination of behaviors has not been determined. This cross-sectional study simultaneously examined the associations between lifestyle factors (fitness, activity behaviors, and dietary patterns) and CMD risk in preadolescent children.

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Lifestyle factors contribute to childhood obesity risk, however it is unclear which lifestyle factors are most strongly associated with childhood obesity. The purpose of this cross-sectional study was to simultaneously investigate the associations among dietary patterns, activity behaviors, and physical fitness with adiposity (body fat %, fat mass, body mass index [BMI], and waist to hip ratio) in preadolescent children. Preadolescent children (N = 392, 50% female, age: 9.

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Acute kidney injury (AKI) is defined as an abrupt decline in glomerular filtration rate, with increased serum creatinine and nitrogenous waste products due to several possible etiologies. Incidence in the pediatric population is estimated to be 3.9 per 1,000 hospitalizations, and prevalence among children admitted to intensive care units is 26.

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Childhood cardiometabolic disease risk (CMD) has been associated with short sleep duration. Its relationship with other aspects of sleep should also be considered, including social jetlag (SJL) which represents the difference between a person's social rhythms and circadian clock. This study investigated whether childhood CMD risk is associated with sleep duration, sleep disturbances, and SJL.

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Objective: To determine the associations between cardiorespiratory fitness (CRF) and fatness (overweight-obesity) with cardiometabolic disease risk among preadolescent children.

Study Design: This cross-sectional study recruited 392 children (50% female, 8-10 years of age). Overweight-obesity was classified according to 2007 World Health Organization criteria for body mass index.

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This cross-sectional study determined whether 6-n-propylthiouracil (PROP) taster status moderates the relationship between food consumption patterns and body composition in children. Children were recruited ( = 342, 50% female, 8-10 y) from across New Zealand. Using a food frequency questionnaire, these food consumption patterns were derived: Processed Foods, Fruit and Vegetables, and Breakfast Foods.

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Background: While short and poor quality sleep have been associated with childhood obesity, no known studies have examined social jetlag. Social jetlag is the discrepancy between an individual's circadian clock and social rhythms, and is measured as the difference in hours between the midpoint of sleep during work/school days and on free (weekend) days. This study investigated the independent associations between sleep duration, sleep disturbances, and social jetlag with adiposity in children.

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Background: Atherosclerosis begins during preadolescence and is occurring at an accelerated rate. This acceleration has been linked to poor lifestyle behaviors and subsequent cardiometabolic complications. Although the clustering of cardiometabolic risk factors has been recognized for over 2 decades, previous studies in children have predominantly examined the relationships between atherosclerosis and individual cardiometabolic risk factors or have grouped together preadolescent and adolescent children.

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Introduction: Although cardiovascular disease is typically associated with middle or old age, the atherosclerotic process often initiates early in childhood. The process of atherosclerosis appears to be occurring at an increasing rate, even in pre-adolescents, and has been linked to the childhood obesity epidemic. This study will investigate the relationships between obesity, lifestyle behaviours and cardiometabolic health in pre-pubescent children aged 8-10 years, and investigates whether there are differences in the correlates of cardiometabolic health between Māori and Caucasian children.

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Article Synopsis
  • Cerebral cavernous malformations (CCMs) are vascular lesions in the central nervous system that can occur sporadically or through inherited mutations in genes KRIT1, CCM2, and PDCD10.
  • Biallelic somatic mutations in CCM lesions support the idea that both inherited and sporadic cases may follow a two-hit mutation mechanism, although the genetic basis for sporadic cases isn't fully established.
  • The study identified novel somatic mutations in sporadic CCM and indicated a shared pathogenic pathway in both inherited and sporadic forms, suggesting potential for similar therapeutic approaches.
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