A thermodynamic bottleneck in the TCA cycle contributes to acetate overflow in .

During aerobic growth, relies on acetate overflow metabolism, a process where glucose is incompletely oxidized to acetate, for its bioenergetic needs. Acetate is not immediately captured as a carbon source and is excreted as waste by cells. The underlying factors governing acetate overflow in have not been identified.

Enzyme-constrained Metabolic Model of Identified Glycerol-3-phosphate Dehydrogenase as an Alternate Electron Sink.

, the causative agent of syphilis, poses a significant global health threat. Its strict intracellular lifestyle and challenges in cultivation have impeded detailed metabolic characterization. In this study, we present iTP251, the first genome-scale metabolic model of , reconstructed and extensively curated to capture its unique metabolic features.

A thermodynamic bottleneck in the TCA cycle contributes to acetate overflow in .

During aerobic growth, relies on acetate overflow metabolism, a process where glucose is incompletely oxidized to acetate, for its bioenergetic needs. Acetate is not immediately captured as a carbon source and is excreted as waste by cells. The underlying factors governing acetate overflow in have not been identified.

Metabolic model guided CRISPRi identifies a central role for phosphoglycerate mutase in persistence.

Unlabelled: Upon nutrient starvation, serovar L2 (CTL) shifts from its normal growth to a non-replicating form, termed persistence. It is unclear if persistence reflects an adaptive response or a lack thereof. To understand this, transcriptomics data were collected for CTL grown under nutrient-replete and nutrient-starved conditions.

Optimal Protein Allocation Controls the Inhibition of GltA and AcnB in .

(Ngo) is a major concern for global public health due to its severe implications for reproductive health. Understanding its metabolic phenotype is crucial for comprehending its pathogenicity. Despite Ngo's ability to encode TCA cycle proteins, GltA and AcnB, their activities are notably restricted.

A multi-organ maize metabolic model connects temperature stress with energy production and reducing power generation.

Climate change has adversely affected maize productivity. Thereby, a holistic understanding of metabolic crosstalk among its organs is important to address this issue. Thus, we reconstructed the first multi-organ maize metabolic model, ZMA6517, and contextualized it with heat and cold stress transcriptomics data using expression distributed reaction flux measurement (EXTREAM) algorithm.

The Inherited Intestinal Microbiota from Myeloid-Specific ZIP8KO Mice Impairs Pulmonary Host Defense against Pneumococcal Pneumonia.

Intestinal dysbiosis increases susceptibility to infection through the alteration of metabolic profiles, which increases morbidity. Zinc (Zn) homeostasis in mammals is tightly regulated by 24 Zn transporters. ZIP8 is unique in that it is required by myeloid cells to maintain proper host defense against bacterial pneumonia.

Supplementation of Sulfide or Acetate and 2-Mercaptoethane Sulfonate Restores Growth of the Δ Deletion Mutant during Methylotrophic Methanogenesis.

Methanogenic archaea are important organisms in the global carbon cycle that grow by producing methane gas. is a methanogenic archaeum that can grow using methylated compounds, carbon monoxide, or acetate and produces renewable methane as a byproduct. However, there is limited knowledge of how combinations of substrates may affect metabolic fluxes in methanogens.

Opening the door to nitrogen fixation in oxygenic phototrophs.

Conveying biological nitrogen fixation (BNF) to photosynthetic species may be the next agricultural revolution, yet poses major engineering challenges. Liu et al. created a diazotrophic strain of a previously non-nitrogen-fixing species, the cyanobacterium Synechocystis sp.

Characterizing the Interplay of Rubisco and Nitrogenase Enzymes in Anaerobic-Photoheterotrophically Grown Rhodopseudomonas palustris CGA009 through a Genome-Scale Metabolic and Expression Model.

Rhodopseudomonas palustris CGA009 is a Gram-negative purple nonsulfur bacterium that grows phototrophically by fixing carbon dioxide and nitrogen or chemotrophically by fixing or catabolizing a wide array of substrates, including lignin breakdown products for its carbon and fixing nitrogen for its nitrogen requirements. It can grow aerobically or anaerobically and can use light, inorganic, and organic compounds for energy production. Due to its ability to convert different carbon sources into useful products during anaerobic growth, this study reconstructed a metabolic and expression (ME) model of R.

Dissecting the metabolic reprogramming of maize root under nitrogen-deficient stress conditions.

The growth and development of maize (Zea mays L.) largely depends on its nutrient uptake through the root. Hence, studying its growth, response, and associated metabolic reprogramming to stress conditions is becoming an important research direction.

Enhancing in silico strain design predictions through next generation metabolic modeling approaches.

The reconstruction and analysis of metabolic models has garnered increasing attention due to the multitude of applications in which these have proven to be practical. The growing number of generated metabolic models has been accompanied by an exponentially expanding arsenal of tools used to analyze them. In this work, we discussed the biological relevance of a number of promising modeling frameworks, focusing on the questions and hypotheses each method is equipped to address.

COVID-19 Antibody Test/Vaccination Certification: There's an App for That.

As the Coronavirus Pandemic of 2019/2020 unfolds, a COVID-19 'Immunity Passport' has been mooted as a way to enable individuals to return back to work. While the quality of antibody testing, the availability of vaccines, and the likelihood of even attaining COVID-19 immunity continue to be researched, we address the issues involved in providing tamper-proof and privacy-preserving certification for test results and vaccinations. We developed a prototype mobile phone app and requisite decentralized server architecture that facilitates instant verification of tamper-proof test results.

COVID-19 Contact Tracing: Challenges and Future Directions.

Contact tracing has become a vital tool for public health officials to effectively combat the spread of new diseases, such as the novel coronavirus disease COVID-19. Contact tracing is not new to epidemiologist rather, it used manual or semi-manual approaches that are incredibly time-consuming, costly and inefficient. It mostly relies on human memory while scalability is a significant challenge in tackling pandemics.