Comparing CIDR with progesterone injections and eCG with human recombinant FSH for synchronizing estrous cycle in ewes.

Background: It is desirable in estrus synchronization in sheep to avoid intravaginal devices and to shorten the program from 14 to 6 days. Moreover, replacement of eCG with safe, cheap, and efficient gonadotropin is in worldwide demand.

Aims: This study investigates the possibility of replacing eCG with human recombinant FSH (hrFSH) and CIDR with progesterone injections for estrous synchronization in ewes.

A preliminary report comparing the effect of Asafoetida with oral contraceptive on polycystic ovarian syndrome in a double-blind randomized trial.

Objective: Polycystic ovarian syndrome (PCOS) is the most common cause of infertility and endocrine disorders in women of childbearing age. In Persian medicine, L. (Asafoetida) was recommended for treating PCOS.

Effect of Intra-ovarian Injection of Mesenchymal Stem Cells or its Conditioned Media on Repeated OPU-IVEP Outcomes in Jersey Heifers and Its Relationship with Follicular Fluid Inflammatory Markers.

Background: Repeated Ovum Pick Up (OPU) could have a detrimental effect on ovarian function, reducing Embryo Production (IVEP). The present study examined the therapeutic effect of adipose-derived Mesenchymal Stem Cells (MSCs) or its Conditioned Medium (ConM) on ovarian trauma following repeated OPU. Resolvin E1 (RvE1) and Interleukin-12 (IL-12) were investigated as biomarkers.

Camel semen collection, viscosity, and cryopreservation: a review.

Artificial insemination in camels remains undeveloped due to the difficulties in semen collection, semen viscosity, and semen cryopreservation. The semen collection procedure has been facilitated to some extent using camel phantom and/or possibly an intravaginal condom. Main reasons for semen viscosity in camelids have been unraveled and different mechanical and enzymatic approaches were used to alleviate this problem; however, there is still no conclusive protocol to safely remove semen viscosity completely.

The effect of varying concentrations of chicken plasma egg yolk and glycerol on the viability of canine sperm following short and long-term preservation.

Background: Plasma egg yolk (PEY), due to simple preparation and easier access, could be a suitable alternative to raw egg yolk for preserving canine semen.

Aims: The present study investigated suitable concentrations of PEY and glycerol for preservation of canine semen.

Methods: Semen was collected by digital manipulation (seven replicates from four dogs).

Synchronization of estrus using progesterone injections followed by human menopausal gonadotropin in ewes.

Serial progesterone injections followed by human menopausal gonadotropin (hMG), instead of equine chorionic gonadotropin (eCG), were used to synchronize estrus in ewes. Shal ewes (n = 189) were assigned into five groups and each group was divided into two sub-groups to receive gonadotropins including eCG (300 IU; intra-muscular) or hMG (one ampoule; subcutaneously, SC). All ewes received prostaglandin (PG) Fα six days after introducing ram (day 0).

Metabolic enhancement of the one carbon metabolism (OCM) in bovine oocytes IVM increases the blastocyst rate: evidences for a OCM checkpoint.

The one carbon metabolism (OCM) has a primary role in the process of oocyte maturation. In this study bovine oocytes were cultured for 24 h, up to MII stage, with standard medium supplemented or not with 8 metabolic enhancers of the OCM and the MII and blastocyst rate were compared. Additional analyses were performed on matured oocytes, cumulus cells, zygotes and blastocysts.

Superovulation of high-producing Holstein lactating dairy cows with human recombinant FSH and hMG.

Superovulation of high-producing dairy cows is a challenging subject in dairy farms with respect to the cost, dose and type of gonadotropin. The objectives of this study were to compare three gonadotropin products: Folltropin-V® (highly purified FSH with porcine origin), Cinnal-f® (recombinant human FSH) and Menotropins® (hMG) for superovulation in high-producing Holstein lactating dairy cows and to investigate the pregnancy outcomes achieved following transferring embryos recovered from donors treated with different gonadotropins. Healthy high-producing Holstein lactating dairy cows (n = 30; milk production: 46.

Size and number of corpora lutea and serum progesterone concentrations when administering two doses of eCG in an estrous synchronization treatment regimen for dairy cattle.

There was investigation of whether there were ovulations from co-dominant follicles following eCG administration. In all experiments, there was GnRH injection and CIDR insertion on day 0 (D0), CIDR withdrawal on D8, and cloprostenol administration on D8 (Exp. I and II) or D7 and D8 (Exp.

Epigenotoxic Effect of Dimethyl Sulfoxide on Buffalo Somatic Cells and Buffalo-Bovine Interspecies Somatic Cell Nuclear Transfer Embryos.

Objective: In the present study, we investigated the possible epigenotoxic effect of dimethyl sulfoxide (DMSO) on buffalo fibroblast cells and on reconstructed oocytes during buffalo-bovine interspecies somatic cell nuclear transfer (iSCNT) procedure and its effect on rate and quality of blastocyst which derived from these reconstructed oocytes.

Materials And Methods: In this experimental study, cell viability of buffalo fibroblasts was assessed after exposure to various concentration (0.5, 1, 2 and 4%) of DMSO using MTS assay.

Effect of DNA and Histone Methyl Transferase Inhibitors on Outcomes of Buffalo-Bovine Interspecies Somatic Cell Nuclear Transfer.

Somatic cell nuclear transfer (SCNT) derived embryos suffer from abnormal epigenetic reprogramming, which handicaps pre- and postimplantation development. It was hypothesized that epigenetic modifiers, including zebularine (DNA methyltransferase inhibitors) and BIX-01294 (histone methyltransferase inhibitors), could decrease the respective levels of 5-methylcytosine and H3K9me2 in reconstructed oocytes (RO). Accordingly, we investigated whether treating RO with zebularine and BIX-01294 for 16 hours after activation could improve developmental competence and quality of buffalo-bovine interspecies SCNT (iSCNT) embryos.

Effect of time of eCG administration on the fate of ovarian follicle in Holstein heifers.

The objective of this study was to investigate the effect of equine chorionic gonadotropin (eCG) on ovarian follicles at three stages of development (emergence, dominance and early static phases) during the first follicular wave (FFW) in Holstein heifers. Heifers (n=20) were randomly assigned into four experimental groups (n=5 in each group). Heifers received eCG (500 IU; Folligon; Intervet, Holland; i.

Comparing three superovulation protocols in dromedary camels: FSH, eCG-FSH and hMG.

The objective of this study was to compare three superovulation protocols in dromedary camels. Follicular waves of dromedary camel donors (n=6) were synchronized using two GnRH injections. Superovulation was initiated 2 days after the second injection (day 0 of experiment).

Superovulatory response following FSH, eCG-FSH and hMG and pregnancy rates following transfer of hatched blastocyst embryos with different diameter and shape in dromedary camel.

The objectives of this study were to compare three superovulation protocols and to investigate the effect of embryo diameter and shape on pregnancy rates in dromedary camel. In Experiment 1, following follicular wave synchronization of donors using two injections of GnRH (25 μg, im), superovulation was initiated 2 days after the second GnRH (Day 0 of experiment). Then, donors were assigned into three groups (n = 5) and received 1) FSH alone (390 mg), 2) eCG (1000 IU; single dose on Day 0) and FSH (330 mg) and 3) hMG (17.

Supplementation of tris-based extender with plasma egg yolk of six avian species and camel skim milk for chilled preservation of dromedary camel semen.

The present study was conducted to investigate a suitable source (Expt. 1) and concentration (Expt. 2) of plasma egg yolk (PEY) and concentration of camel skim milk (CSM; Expt.

Clinical and paraclinical evaluation of partial nephrectomy using laparoscopy and open surgery in dogs: new suturing technique.

Partial nephrectomy, using open surgery or laparoscopy, is a standard surgical approach to treat renal disorders. The objective of this study was to compare and evaluate the feasibility and safety of laparoscopic partial nephrectomy using figure eight ligation technique. Mixed-breed dogs were randomly dedicated for partial nephrectomy using laparoscopy (n=6) and open surgery (n=6).

Comparison of virulence factors and biofilm formation among Staphylococcus aureus strains isolated from human and bovine infections.

The aim of this study was to find different prevalence of genes involved in the biofilm formation process and to assess the phenotypic and genotypic markers of biofilm formation among Staphylococcus aureus strains isolated from human and bovine infections. In this study, 215 S. aureus strains were collected from human and dairy cow's infections.

Effect of camel milk on blood sugar and lipid profile of patients with type 2 diabetes: a pilot clinical trial.

Background: It has been shown that camel milk consumption has a definite decreasing effect on the prevalence of diabetes. However, most of these studies were conducted on patients with type 1 diabetes, whereas studies on patients with type 2 diabetes mellitus (T2DM) are limited. In vitro experiments have shown that camel milk was able to decrease blood glucose concentration.

Comparison between two portal laparoscopy and open surgery for ovariectomy in dogs.

Ovariectomy (OVE) is a routine surgical procedure for neutering in small animal practice. Laparoscopy is a new surgical technique which contains advantages such as less trauma, smaller incision and excellent visualization than traditional open surgery. The present study was conducted to examine the feasibility and safety of laparoscopic procedure through two portal comparing with the conventional open surgery for OVE in healthy female bitches (n=16).

Semen collection using phantom in dromedary camel.

Semen collection is relatively long, unsafe, and tedious procedure in dromedary camel. The innovation of safe, hygienic, and simple approach to collect semen could make great progress in development of AI program in this species. This study investigated two methods of semen collection using phantom and artificial vagina in dromedary camel.

Parallel distribution of sexes within left and right uterine horns in Holstein dairy cows: evidence that the effect of side of pregnancy on sex ratio could be breed-specific in cattle.

Dissimilar distribution of male and female calves within left and right uterine horns has been observed in beef cows. A retrospective study was conducted to investigate the effect of side of pregnancy on secondary sex ratio in Holstein dairy cows. Data associated with sex of calves, side of pregnancy, sire, dam, parity number of dam, AI technician, season and year were retrieved from the database of a Holstein dairy farm.

Administration of eCG on Day 6 postpartum could enhance reproductive performance of Holstein dairy cows.

Injection of eCG on Day 6 postpartum could enhance early resumption of ovarian activity in Holstein dairy cows. The present study was conducted to investigate the effects of eCG treatment on Day 6 postpartum on reproductive performance of Holstein dairy cows. Holstein dairy cows (n=420) were randomly assigned to two groups.

Heat-induced conformational unfolding of fatty acid-free and fatted camel serum albumin: a comparative study.

Albumin is a multifunctional non-glycosylated, negatively charged plasma protein, with extraordinary ligand-binding and transport properties, antioxidant functions, and enzymatic activities. Physiologically, albumin transports free fatty acids in plasma and contributes in maintaining colloid osmotic pressure. Recent progresses in using albumin as a versatile protein carrier for drug targeting and for improving the pharmacokinetic profile of peptide or protein-based drugs, increased the attempts for improving albumin stability.

Comparative evaluation of two reconstructive methods following laparoscopic assisted subtotal gastrectomy in dogs.

Background: Laparoscopic gastrectomy is a new and technically challenging surgical procedure with potential benefit. The objective of this study was to investigate clinical and para-clinical consequences following Roux-en-Y and Jejunal Loop interposition reconstructive techniques for subtotal gastrectomy using laparoscopic assisted surgery.

Results: Following resection of the stomach attachments through a laparoscopic approach, stomach was removed and reconstruction was performed with either standard Roux-en-Y (n = 5) or Jejunal Loop interposition (n = 5) methods.

Comparison between single and three portal laparoscopic splenectomy in dogs.

Background: Single incision laparoscopic surgery (SILS) is a newly growing technique to replace a more invasive conventional multiple portal laparoscopic surgery. The objective of this study was to compare single (SILS) with three portal (Conventional) laparoscopic splenectomy in dogs. Mongrel dogs (n = 18), weighting 15 ± 3 kg, were selected for this study (n = 12 SILS; n = 6 conventional).