Response of soil microbial community structure to temperature and nitrogen fertilizer in three different provenances of .

Soil microorganisms are key indicators of soil health, and it is crucial to investigate the structure and interactions of soil microbial communities among three different provenances of under varying nitrogen fertilizer and temperature levels in Northwest China. This study aims to provide theoretical support for the sustainable use of artificial grassland in this region. Employing a two-factor pot-control experiment with three nitrogen fertilizer treatments and three temperature treatments, a total of all treatments was utilized to examine the composition and abundance of soil microbial communities associated with using high-throughput sequencing, PCR technology, and molecular ecological network analysis.