Evaluation of antifungal activities and structure-activity relationships of coumarin derivatives.

Background: Osthol is a natural coumarin and lead compound that has been developed into commercial fungicides in China. Natural coumarins comprise five major subtypes: simple coumarins, linear furanocoumarins, angular furanocoumarins, linear pyranocoumarins and angular pyranocoumarins. Studies pertaining to the antifungal activities of linear pyranocoumarins are few, and no reports exist for the antifungal activities of angular pyranocoumarins.

[Chemical constituents of Angelica nitida roots].

Objective: To investigate the chemical constituents in the roots of Angelica nitida.

Methods: The chemical constituents were isolated by silica gel,their structures were identified by spectral analysis.

Results: Nine compounds were isolated and identified as isoimperatorin(I), imperatorin(II), cnidilin(III), beta-sitosterol(IV), isopimpinellin(V), phellopterin(VI), neobyakangelicol(VII), (3S)-2,2-dimethyl-3,5-dihydroxy-8-hydroxylmethyl-3,4-dihydro-2H,6H-benzo[1,2-b: 5,4-b'] dipyran-6-one(VIll) and byakangelicin (IX).

Identification of species and materia medica within Angelica L. (Umbelliferae) based on phylogeny inferred from DNA barcodes.

DNA barcodes have been increasingly used in authentication of medicinal plants, while their wide application in materia medica is limited in their accuracy due to incomplete sampling of species and absence of identification for materia medica. In this study, 95 leaf accessions of 23 species (including one variety) and materia medica of three Pharmacopoeia-recorded species of Angelica in China were collected to evaluate the effectiveness of four DNA barcodes (rbcL, matK, trnH-psbA and ITS). Our results showed that ITS provided the best discriminatory power by resolving 17 species as monophyletic lineages without shared alleles and exhibited the largest barcoding gap among the four single barcodes.

[Studies on the chemical constituents in roots of Angelica tianmuensis and A. megaphylla].

Objective: To study the chemical constituents in roots of Angelica tianmuensis and A. megaphylla.

Methods: Compounds were isolated by column chromatography with silica gel, their structures were identified by spectral analysis.

Five condensed furanocoumarins from the root of Heracleum candicans Wall.

Three new spirotrifuranocoumarins, canditririns C-E (1-3), a new spirotetrafuranocoumarin, canditetrarin A (4), and a new tetrafuranocoumarin, canditetrarin B (5), were isolated from the roots of Heracleum candicans Wall. Their structures were established using spectral methods.

Four novel furanocoumarin glucosides, candinosides A, B, C and D, from Heracleum candicans Wall.

Four novel furanocoumarin glucosides, candinosides A, B, C and D (1-4), were isolated from the roots of Heracleum candicans Wall. Their structures were established using chemical and spectral methods.

Chemical studies on the root of Heracleum candicans Wall. (Part 3).

Two new ester coumarins, candinols B and C (1 and 2), and three new coumarin dimers, candibirins F-H (3-5), were isolated from the roots of Heracleum candicans Wall. Their structures were established by using chemical and spectral means.

Chemical studies on the root of Heracleum candicans Wall.

Two new alkyl coumarins, isophellodenol C (1) and candinol A (2); four new spirobifuranocoumarins, candibirins B-E (3-6); and two trifuranocoumarins, canditririns A and B (7 and 8), were isolated from the roots of Heracleum candicans Wall. Their structures were established using chemical and spectral means.

Coumarins from the roots of Ligusticum multivittatum.

Five new coumarins, (+)-peujaponisin (1) and multivittans A-D (2-5), were isolated from the roots of Ligusticum multivittatum Franch., and their structures were established by spectral means.

Two isocoumarins from Pleurospermum angelicoides.

Two new isocoumarins, angelicoins A and B, were isolated from the roots of Pleurospermum angelicoides, and their structures were established by spectral means.

Bisabolane-type sesquiterpenes: liginvolones A-D from ligusticum involucratum.

Four new bisabolane-type sesquiterpenes, liginvolones A-D (1-4), a new coumarin, 4'-octanoyloxyosthenol (5), and 20 known constituents were isolated from Ligusticum involucratum. Their structures were elucidated on the basis of spectroscopic analyses and chemical methods.

Four coumarins from Heracleum yunngningense.

Four new coumarins (1-4) were isolated from the roots of Heracleum yunngningense HAND.-MASS. Their structures were established by spectral analyses.

Candibirin A, a furanocoumarin dimer isolated from Heracleum candicans WALL.

Candibirin A [systematic name: 9,9'-(1,4-dioxane-2,5-diyldimethylenedioxy)di(7H-furo[3,2-g]chromen-7-one)], a new furanocoumarin dimer, was isolated from Heracleum candicans WALL. (1)H NMR and MS spectra had indicated that the title compound was a dimer of heraclenin or heraclenol, but the linkage structure and its chirality were undetermined. The dioxane linkage, having the R,R configuration, has now been elucidated from dimethyl sulfoxide-solvated crystals, C(32)H(28)O(10).

Sinodielides E-H, four new guaianolides, from the root of Sinodielsia yunnanensis.

Four new guaianolides, sinodielides E-H (1-4), were isolated from the root of Sinodielsia yunnanensis WOLFF. Their structures were established by spectral evidence.

Four guaianolides from Sinodielsia yunnanensis.

Four new guaianolides, sinodielides A-D (1-4), were isolated from Sinodielsia yunnanensis WOLFF together with a known polyacetylene, falcarindiol, and two known coumarins, bergapten and scopoletin. Their structures were established by spectral and X-ray analyses.